Detailed information of TA system TA073245

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1141245..1142043
• Replicon: chromosome
• Accession: NZ_CP019777
• Organism: Escherichia coli NU14

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1RDD2
• Locus tag: B0908_RS05745
• Protein ID: WP_001094434.1
• Coordinates: 1141669..1142043 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: B0908_RS05740
• Protein ID: WP_001552747.1
• Coordinates: 1141245..1141622 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0908_RS05710	1138123..1138944	+	822	WP_001234585.1	DUF945 domain-containing protein	-
B0908_RS05715	1138944..1139189	+	246	WP_001164966.1	hypothetical protein	-
B0908_RS05720	1139283..1139756	+	474	WP_001542276.1	antirestriction protein	-
B0908_RS05725	1139772..1140248	+	477	WP_001186727.1	RadC family protein	-
B0908_RS05730	1140311..1140532	+	222	WP_000692315.1	DUF987 domain-containing protein	-
B0908_RS05735	1140551..1141195	+	645	WP_000086759.1	hypothetical protein	-
B0908_RS05740	1141245..1141622	+	378	WP_001552747.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
B0908_RS05745	1141669..1142043	+	375	WP_001094434.1	TA system toxin CbtA family protein	Toxin
B0908_RS05750	1142040..1142531	+	492	WP_000976859.1	hypothetical protein	-
B0908_RS05755	1142543..1142740	+	198	WP_000839257.1	DUF957 domain-containing protein	-
B0908_RS05760	1142833..1143675	+	843	WP_001280460.1	DUF4942 domain-containing protein	-
B0908_RS05770	1144146..1145084	+	939	WP_000351298.1	glyoxylate/hydroxypyruvate reductase GhrA	-
B0908_RS05775	1145139..1145876	+	738	WP_000283664.1	zinc-binding phosphatase	-
B0908_RS05780	1145900..1146454	+	555	WP_001001909.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgG / csgF / csgE / csgD / csgB / csgA / csgC	1121119..1151984	30865

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13921.82 Da
• Isoelectric Point: 6.8517

>T73245 WP_001094434.1 NZ_CP019777:1141669-1142043 [Escherichia coli NU14]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T73245 NZ_CP019777:1141669-1142043 [Escherichia coli NU14]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13757.63 Da
• Isoelectric Point: 6.3127

>AT73245 WP_001552747.1 NZ_CP019777:1141245-1141622 [Escherichia coli NU14]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRTGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT73245 NZ_CP019777:1141245-1141622 [Escherichia coli NU14]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCACCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCTGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCACCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: Q1RDD2