Detailed information of TA system TA073233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2066875..2067509
• Replicon: chromosome
• Accession: NZ_CP019732
• Organism: Pseudomonas syringae pv. actinidiae strain CRAFRU 14.08

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B1F85_RS10210
• Protein ID: WP_003380365.1
• Coordinates: 2066875..2067279 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q888H8
• Locus tag: B1F85_RS10215
• Protein ID: WP_003380366.1
• Coordinates: 2067279..2067509 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1F85_RS10175	2062305..2062787	+	483	WP_003380358.1	PAS domain-containing protein	-
B1F85_RS10180	2062883..2064139	+	1257	WP_003380359.1	3-phosphoshikimate 1-carboxyvinyltransferase	-
B1F85_RS10185	2064204..2064440	+	237	WP_003380360.1	hypothetical protein	-
B1F85_RS10190	2064516..2065178	-	663	WP_003380361.1	ChrR family anti-sigma-E factor	-
B1F85_RS10195	2065178..2065690	-	513	WP_003380362.1	sigma-70 family RNA polymerase sigma factor	-
B1F85_RS10200	2065926..2066114	-	189	WP_003380363.1	hypothetical protein	-
B1F85_RS10205	2066113..2066688	+	576	WP_019334082.1	hypothetical protein	-
B1F85_RS10210	2066875..2067279	-	405	WP_003380365.1	tRNA(fMet)-specific endonuclease VapC	Toxin
B1F85_RS10215	2067279..2067509	-	231	WP_003380366.1	antitoxin	Antitoxin
B1F85_RS10220	2067624..2068505	-	882	WP_003380368.1	aldose 1-epimerase	-
B1F85_RS10225	2068480..2069450	-	971	Protein_1892	DMT family transporter	-
B1F85_RS10230	2069534..2070814	-	1281	WP_003380372.1	TRAP transporter large permease	-
B1F85_RS10235	2070815..2071342	-	528	WP_003380373.1	TRAP transporter small permease	-
B1F85_RS10240	2071406..2072445	-	1040	Protein_1895	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-34)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14814.09 Da
• Isoelectric Point: 6.8615

>T73233 WP_003380365.1 NZ_CP019732:c2067279-2066875 [Pseudomonas syringae pv. actinidiae]
MLKYMLDTNICIFTIKNKPVSVREAFNLHHGQLCISAITLMELVYGAEKSSSPERNLAVVEGFAARLELLPYDSAAAAHT
GMIRAELARAGTPIGPYDQMIAGHARSLGLVVITNNQREFQRVEGLRVEDWVSQ

Nucleotide

• Length: 405 bp

>T73233 NZ_CP019732:c2067279-2066875 [Pseudomonas syringae pv. actinidiae]
ATGCTCAAATACATGCTCGACACCAATATTTGCATCTTCACTATCAAGAACAAGCCGGTGTCGGTACGTGAGGCCTTCAA
CCTTCATCACGGGCAGCTGTGTATCAGCGCGATCACGTTGATGGAGCTTGTTTACGGCGCAGAAAAGTCTTCCAGTCCCG
AAAGAAATCTTGCTGTTGTAGAAGGTTTTGCAGCGCGTCTTGAATTGCTTCCCTACGATAGCGCCGCCGCGGCTCACACC
GGCATGATCAGAGCCGAGCTGGCCAGAGCGGGTACACCGATTGGCCCGTACGATCAAATGATTGCAGGACATGCCCGCTC
TCTTGGCCTCGTGGTCATCACCAACAACCAGCGTGAATTCCAGCGCGTCGAGGGGCTGAGGGTAGAAGACTGGGTAAGCC
AATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8593.67 Da
• Isoelectric Point: 4.5557

>AT73233 WP_003380366.1 NZ_CP019732:c2067509-2067279 [Pseudomonas syringae pv. actinidiae]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAEREQPVEQLRESL

Nucleotide

• Length: 231 bp

>AT73233 NZ_CP019732:c2067509-2067279 [Pseudomonas syringae pv. actinidiae]
ATGGAACAATCCACAGTCTTCAAAAGCAATCGAAGTCAGGCTGTTCGACTCCCAAAAGCGGTGGCATTACCCGACGACGT
GAAACGGGTAGACGTCGTAGCAGTGGGCAGGACACGCATCATTTCCCCTGCTGGTGAAATGTGGAACAGTTGGTTCGATG
GTGAAAGTGTCAGTGATGACTTCATGGCAGAGCGCGAGCAGCCAGTTGAACAGCTGCGCGAATCACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0K8LU44