Detailed information of TA system TA073176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 880908..881635
• Replicon: chromosome
• Accession: NZ_CP019689
• Organism: Shigella sonnei strain 75/02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: BZ172_RS04770
• Protein ID: WP_000547563.1
• Coordinates: 881324..881635 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BZ172_RS04765
• Protein ID: WP_000126296.1
• Coordinates: 880908..881327 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZ172_RS04745 (875959)	875959..876486	-	528	WP_001078766.1	electron transport protein HydN	-
BZ172_RS04750 (876634)	876634..877644	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
BZ172_RS04755 (877904)	877904..879361	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
BZ172_RS04760 (879370)	879370..880794	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
BZ172_RS04765 (880908)	880908..881327	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
BZ172_RS04770 (881324)	881324..881635	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
BZ172_RS04775 (881797)	881797..882570	-	774	WP_001026459.1	hypothetical protein	-
BZ172_RS04780 (882595)	882595..883065	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
BZ172_RS04785 (883058)	883058..883468	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
BZ172_RS04790 (883465)	883465..884232	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
BZ172_RS04795 (884232)	884232..884774	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
BZ172_RS04800 (884784)	884784..886481	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T73176 WP_000547563.1 NZ_CP019689:c881635-881324 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T73176 NZ_CP019689:c881635-881324 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT73176 WP_000126296.1 NZ_CP019689:c881327-880908 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT73176 NZ_CP019689:c881327-880908 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22