Detailed information of TA system TA072135

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 86869..87139
• Replicon: plasmid p13P460A-1
• Accession: NZ_CP019272
• Organism: Escherichia coli strain 13P460A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BWI88_RS25085
• Protein ID: WP_001312861.1
• Coordinates: 86981..87139 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	86869..86932 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI88_RS25060	82580..83107	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
BWI88_RS25065	83165..83398	+	234	WP_000006003.1	DUF905 family protein	-
BWI88_RS25070	83459..85482	+	2024	Protein_101	ParB/RepB/Spo0J family partition protein	-
BWI88_RS25075	85551..85985	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
BWI88_RS25080	85982..86701	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	86713..86937	+	225	NuclAT_0	-	-
-	86713..86937	+	225	NuclAT_0	-	-
-	86713..86937	+	225	NuclAT_0	-	-
-	86713..86937	+	225	NuclAT_0	-	-
BWI88_RS26670	86722..86901	-	180	WP_001309233.1	hypothetical protein	-
-	86869..86932	-	64	-	-	Antitoxin
BWI88_RS25085	86981..87139	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BWI88_RS26845	87377..87754	-	378	Protein_106	hypothetical protein	-
BWI88_RS25105	88054..88350	+	297	WP_001272251.1	hypothetical protein	-
BWI88_RS25110	88461..89282	+	822	WP_001234445.1	DUF945 domain-containing protein	-
BWI88_RS25115	89579..90226	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
BWI88_RS25120	90503..90886	+	384	WP_000124981.1	relaxosome protein TraM	-
BWI88_RS25125	91077..91763	+	687	WP_015059009.1	PAS domain-containing protein	-
BWI88_RS25130	91857..92084	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / oqxA / oqxB / aph(3')-IIa / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..129452	129452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T72135 WP_001312861.1 NZ_CP019272:86981-87139 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T72135 NZ_CP019272:86981-87139 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT72135 NZ_CP019272:c86932-86869 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin