Detailed information of TA system TA071965

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 33518..33782
• Replicon: plasmid pCombat13F7-3
• Accession: NZ_CP019248
• Organism: Escherichia coli strain Combat13F7

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: BWI83_RS25795
• Protein ID: WP_001331364.1
• Coordinates: 33518..33670 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	33720..33782 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI83_RS25775	28786..30954	+	2169	WP_000698354.1	DotA/TraY family protein	-
BWI83_RS25780	31025..31687	+	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
BWI83_RS25785	31759..31968	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
BWI83_RS26990	32360..32536	+	177	WP_001054897.1	hypothetical protein	-
BWI83_RS25790	33195..33446	+	252	WP_001291964.1	hypothetical protein	-
BWI83_RS25795	33518..33670	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	33720..33782	+	63	NuclAT_0	-	Antitoxin
-	33720..33782	+	63	NuclAT_0	-	Antitoxin
-	33720..33782	+	63	NuclAT_0	-	Antitoxin
-	33720..33782	+	63	NuclAT_0	-	Antitoxin
BWI83_RS25800	33962..35170	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
BWI83_RS25805	35189..36259	+	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
BWI83_RS25810	36252..38543	+	2292	WP_001289276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3'')-Ib / aph(6)-Id	-	1..121975	121975

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T71965 WP_001331364.1 NZ_CP019248:c33670-33518 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T71965 NZ_CP019248:c33670-33518 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT71965 NZ_CP019248:33720-33782 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin