Detailed information of TA system TA071801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 240172..240842
• Replicon: plasmid pGN-2
• Accession: NZ_CP019161
• Organism: Klebsiella pneumoniae strain GN-2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: BKY56_RS28215
• Protein ID: WP_004213072.1
• Coordinates: 240399..240842 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: BKY56_RS28210
• Protein ID: WP_004213073.1
• Coordinates: 240172..240402 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKY56_RS28185	236395..237294	-	900	WP_004225022.1	nucleotide-binding protein	-
BKY56_RS28190	237284..237574	-	291	WP_004213078.1	hypothetical protein	-
BKY56_RS28195	237926..238132	+	207	WP_004213077.1	hypothetical protein	-
BKY56_RS28200	238122..238415	-	294	WP_004213076.1	hypothetical protein	-
BKY56_RS28205	238431..239564	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
BKY56_RS28210	240172..240402	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BKY56_RS28215	240399..240842	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
BKY56_RS28220	240856..241272	-	417	Protein_235	restriction endonuclease	-
BKY56_RS28225	241276..241569	-	294	Protein_236	transposase	-
BKY56_RS29105	241986..242624	+	639	Protein_237	response regulator	-
BKY56_RS28245	243139..243545	-	407	Protein_238	GAF domain-containing protein	-
BKY56_RS28255	243636..244556	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
BKY56_RS28260	244605..245096	-	492	WP_011251327.1	DM13 domain-containing protein	-
BKY56_RS28265	245159..245434	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..261986	261986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T71801 WP_004213072.1 NZ_CP019161:240399-240842 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T71801 NZ_CP019161:240399-240842 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT71801 WP_004213073.1 NZ_CP019161:240172-240402 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT71801 NZ_CP019161:240172-240402 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620