Detailed information of TA system TA071704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 8040..8587
• Replicon: plasmid p1493-3
• Accession: NZ_CP019074
• Organism: Escherichia coli strain CRE1493

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BVL38_RS25665
• Protein ID: WP_050858704.1
• Coordinates: 8306..8587 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A829FT18
• Locus tag: BVL38_RS25660
• Protein ID: WP_000079938.1
• Coordinates: 8040..8309 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL38_RS25625	3330..3962	-	633	WP_101973396.1	IS6 family transposase	-
BVL38_RS25635	4229..5665	+	1437	WP_000105383.1	glutathione synthase	-
BVL38_RS25640	6083..7087	+	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
BVL38_RS27845	7286..7570	-	285	WP_058672119.1	Tn3 family transposase	-
BVL38_RS28080	7827..7967	+	141	WP_160784086.1	hypothetical protein	-
BVL38_RS25660	8040..8309	+	270	WP_000079938.1	hypothetical protein	Antitoxin
BVL38_RS25665	8306..8587	+	282	WP_050858704.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BVL38_RS27850	8640..8777	+	138	WP_152931508.1	hypothetical protein	-
BVL38_RS25675	9115..9372	-	258	WP_063072981.1	biofilm development YmgB/AriR family protein	-
BVL38_RS25680	9712..9816	-	105	Protein_12	integrase core domain-containing protein	-
BVL38_RS25685	10011..10436	-	426	WP_063072980.1	ester cyclase	-
BVL38_RS25690	10445..11434	-	990	WP_001541953.1	helix-turn-helix domain-containing protein	-
BVL38_RS25695	11450..12226	-	777	WP_022631027.1	MBL fold metallo-hydrolase	-
BVL38_RS25700	12602..13299	-	698	Protein_16	IS1 family transposase	-
BVL38_RS28305	13386..13454	-	69	WP_211180522.1	protein YkiE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aac(3)-IId / dfrA12 / aadA2 / oqxA / oqxB / tet(A) / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / floR	-	1..73992	73992

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10860.58 Da
• Isoelectric Point: 8.4615

>T71704 WP_050858704.1 NZ_CP019074:8306-8587 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMVAIELDERIGYSASSLAGQPYKGRNGRVEGARELVIHPHFVLVYEVDSPWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T71704 NZ_CP019074:8306-8587 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGTCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTGCCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCCGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10251.64 Da
• Isoelectric Point: 10.1734

>AT71704 WP_000079938.1 NZ_CP019074:8040-8309 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVADK

Nucleotide

• Length: 270 bp

>AT71704 NZ_CP019074:8040-8309 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829FT18