Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5270440..5271065 Replicon chromosome
Accession NZ_CP019047
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJA166

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag RJA_RS26040 Protein ID WP_012737091.1
Coordinates 5270440..5270823 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag RJA_RS26045 Protein ID WP_004150355.1
Coordinates 5270823..5271065 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJA_RS26025 5267806..5268708 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
RJA_RS26030 5268705..5269340 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
RJA_RS26035 5269337..5270266 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
RJA_RS26040 5270440..5270823 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
RJA_RS26045 5270823..5271065 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
RJA_RS26050 5271270..5272187 + 918 WP_012737090.1 alpha/beta hydrolase -
RJA_RS26055 5272201..5273142 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
RJA_RS26060 5273187..5273624 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
RJA_RS26065 5273621..5274481 - 861 WP_004146232.1 virulence factor BrkB family protein -
RJA_RS26070 5274475..5275074 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T71606 WP_012737091.1 NZ_CP019047:c5270823-5270440 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T71606 NZ_CP019047:c5270823-5270440 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT71606 WP_004150355.1 NZ_CP019047:c5271065-5270823 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT71606 NZ_CP019047:c5271065-5270823 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References