Detailed information of TA system TA071364

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57290..57544
• Replicon: plasmid pECAZ155_KPC
• Accession: NZ_CP019001
• Organism: Escherichia coli strain Ecol_AZ155

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BE948_RS00410
• Protein ID: WP_015387399.1
• Coordinates: 57338..57544 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	57290..57351 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE948_RS00360	52942..53154	+	213	WP_005012601.1	hypothetical protein	-
BE948_RS27730	53455..53544	-	90	Protein_65	IS1 family transposase	-
BE948_RS00375	53599..54276	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
BE948_RS00380	54276..54623	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
BE948_RS00385	54643..56214	+	1572	WP_023149734.1	IS66 family transposase	-
BE948_RS00390	56252..56868	-	617	Protein_69	IS1 family transposase	-
BE948_RS00400	56969..57151	+	183	WP_000968309.1	hypothetical protein	-
-	57290..57351	-	62	NuclAT_2	-	Antitoxin
-	57290..57351	-	62	NuclAT_2	-	Antitoxin
-	57290..57351	-	62	NuclAT_2	-	Antitoxin
-	57290..57351	-	62	NuclAT_2	-	Antitoxin
BE948_RS00410	57338..57544	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE948_RS00415	57828..58085	+	258	WP_000083833.1	replication regulatory protein RepA	-
BE948_RS00425	58321..58395	+	75	WP_032336874.1	RepA leader peptide Tap	-
BE948_RS00430	58388..58834	+	447	Protein_74	incFII family plasmid replication initiator RepA	-
BE948_RS00435	58780..59262	-	483	Protein_75	VENN motif pre-toxin domain-containing protein	-
BE948_RS00440	60155..61375	+	1221	WP_000410951.1	arginine deiminase	-
BE948_RS00445	61386..62297	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / sul1 / qacE / aac(6')-Il / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2 / tet(B) / sitABCD	iucA / iucB / iucC / iucD / iutA	1..272202	272202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T71364 WP_015387399.1 NZ_CP019001:57338-57544 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T71364 NZ_CP019001:57338-57544 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT71364 NZ_CP019001:c57351-57290 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin