Detailed information of TA system TA071329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 24643..25286
• Replicon: plasmid pECAZ153_KPC
• Accession: NZ_CP018999
• Organism: Escherichia coli strain Ecol_AZ153

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: BE940_RS00935
• Protein ID: WP_001044770.1
• Coordinates: 24643..25059 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: BE940_RS00940
• Protein ID: WP_001261282.1
• Coordinates: 25056..25286 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE940_RS00910	19943..20647	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BE940_RS00915	20743..21018	-	276	Protein_25	transposase	-
BE940_RS00920	21047..21744	-	698	Protein_26	IS1 family transposase	-
BE940_RS00925	22000..23022	-	1023	WP_000361404.1	helicase UvrD	-
BE940_RS00930	23007..24569	-	1563	WP_004206609.1	AAA family ATPase	-
BE940_RS00935	24643..25059	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
BE940_RS00940	25056..25286	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BE940_RS00945	25243..25704	+	462	WP_160866775.1	hypothetical protein	-
BE940_RS00955	25874..26092	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
BE940_RS00960	26094..26399	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
BE940_RS00965	26568..26963	+	396	WP_017899885.1	hypothetical protein	-
BE940_RS00970	26990..27304	+	315	WP_053389906.1	hypothetical protein	-
BE940_RS00975	27315..28331	+	1017	WP_017899884.1	hypothetical protein	-
BE940_RS00980	28529..29323	+	795	WP_004197635.1	site-specific integrase	-
BE940_RS00985	29803..29982	-	180	WP_006788217.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / dfrA25 / blaTEM-1C / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId / aph(3')-Ia / blaKPC-2	-	1..146162	146162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T71329 WP_001044770.1 NZ_CP018999:c25059-24643 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T71329 NZ_CP018999:c25059-24643 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT71329 WP_001261282.1 NZ_CP018999:c25286-25056 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT71329 NZ_CP018999:c25286-25056 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3