Detailed information of TA system TA071323

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35568..35810
• Replicon: plasmid pECAZ153_2
• Accession: NZ_CP018998
• Organism: Escherichia coli strain Ecol_AZ153

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BE940_RS00300
• Protein ID: WP_001312861.1
• Coordinates: 35568..35726 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	35770..35810 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE940_RS00250	30680..30907	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
BE940_RS00255	30995..31672	-	678	WP_001348626.1	PAS domain-containing protein	-
BE940_RS00260	31806..32189	-	384	WP_001151566.1	relaxosome protein TraM	-
BE940_RS00265	32520..33122	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
BE940_RS00270	33419..34240	-	822	WP_001234469.1	DUF945 domain-containing protein	-
BE940_RS00275	34359..34646	-	288	WP_000107535.1	hypothetical protein	-
BE940_RS28575	34671..34877	-	207	WP_000275859.1	hypothetical protein	-
BE940_RS00300	35568..35726	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	35770..35810	-	41	NuclAT_1	-	Antitoxin
-	35770..35810	-	41	NuclAT_1	-	Antitoxin
-	35770..35810	-	41	NuclAT_1	-	Antitoxin
-	35770..35810	-	41	NuclAT_1	-	Antitoxin
-	37254..37440	-	187	NuclAT_0	-	-
-	37254..37440	-	187	NuclAT_0	-	-
-	37254..37440	-	187	NuclAT_0	-	-
-	37254..37440	-	187	NuclAT_0	-	-
BE940_RS00315	37452..38171	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
BE940_RS00320	38168..38602	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
BE940_RS00325	38657..40615	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sul1 / qacE / aadA5 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A)	-	1..99006	99006

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71323 WP_001312861.1 NZ_CP018998:c35726-35568 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71323 NZ_CP018998:c35726-35568 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT71323 NZ_CP018998:c35810-35770 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin