Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4717017..4717671 Replicon chromosome
Accession NZ_CP018976
Organism Escherichia coli strain Ecol_545

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag BE949_RS25690 Protein ID WP_000244781.1
Coordinates 4717264..4717671 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BE949_RS25685 Protein ID WP_000354046.1
Coordinates 4717017..4717283 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE949_RS25665 4713105..4714538 - 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -
BE949_RS25670 4714583..4714894 + 312 WP_001598596.1 N(4)-acetylcytidine aminohydrolase -
BE949_RS25675 4715058..4715717 + 660 WP_000250269.1 hemolysin III family protein -
BE949_RS25680 4715794..4716774 - 981 WP_001562036.1 tRNA-modifying protein YgfZ -
BE949_RS25685 4717017..4717283 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BE949_RS25690 4717264..4717671 + 408 WP_000244781.1 protein YgfX Toxin
BE949_RS25695 4717711..4718232 - 522 WP_001055874.1 flavodoxin FldB -
BE949_RS25700 4718344..4719240 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
BE949_RS25705 4719265..4719975 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BE949_RS25710 4719981..4721714 + 1734 WP_001598594.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T71174 WP_000244781.1 NZ_CP018976:4717264-4717671 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T71174 NZ_CP018976:4717264-4717671 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT71174 WP_000354046.1 NZ_CP018976:4717017-4717283 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT71174 NZ_CP018976:4717017-4717283 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References