Detailed information of TA system TA071170

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3119095..3119927
• Replicon: chromosome
• Accession: NZ_CP018976
• Organism: Escherichia coli strain Ecol_545

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: BE949_RS17595
• Protein ID: WP_000854765.1
• Coordinates: 3119095..3119469 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: BE949_RS17600
• Protein ID: WP_001295723.1
• Coordinates: 3119559..3119927 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE949_RS27585	3115570..3115728	-	159	WP_001467148.1	hypothetical protein	-
BE949_RS17575	3115828..3116004	-	177	WP_001586605.1	DUF957 domain-containing protein	-
BE949_RS17580	3116021..3116431	-	411	Protein_2931	hypothetical protein	-
BE949_RS17585	3116516..3117262	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
BE949_RS17590	3117277..3118818	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
BE949_RS17595	3119095..3119469	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
BE949_RS17600	3119559..3119927	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BE949_RS17610	3120090..3120311	-	222	WP_000692345.1	DUF987 domain-containing protein	-
BE949_RS17615	3120380..3120856	-	477	WP_001354275.1	RadC family protein	-
BE949_RS17620	3120872..3121345	-	474	WP_001387789.1	antirestriction protein	-
BE949_RS27870	3121511..3121684	-	174	WP_001183321.1	hypothetical protein	-
BE949_RS17630	3121684..3122502	-	819	WP_001234716.1	DUF945 domain-containing protein	-
BE949_RS17640	3122657..3122815	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T71170 WP_000854765.1 NZ_CP018976:c3119469-3119095 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T71170 NZ_CP018976:c3119469-3119095 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT71170 WP_001295723.1 NZ_CP018976:c3119927-3119559 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT71170 NZ_CP018976:c3119927-3119559 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52