Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 23252..23799 Replicon plasmid pEC545_3
Accession NZ_CP018973
Organism Escherichia coli strain Ecol_545

Toxin (Protein)


Gene name parE Uniprot ID A0A4Y1YQH8
Locus tag BE949_RS00210 Protein ID WP_012881118.1
Coordinates 23252..23533 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E2GHF7
Locus tag BE949_RS00215 Protein ID WP_012881119.1
Coordinates 23530..23799 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE949_RS00190 19291..20166 + 876 WP_032277257.1 class A extended-spectrum beta-lactamase CTX-M-27 -
BE949_RS00195 20618..21724 + 1107 Protein_26 maltoporin LamB -
BE949_RS00200 21759..22241 - 483 WP_001311056.1 hypothetical protein -
BE949_RS00205 22358..23206 - 849 WP_012881117.1 3'-5' exonuclease -
BE949_RS00210 23252..23533 - 282 WP_012881118.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BE949_RS00215 23530..23799 - 270 WP_012881119.1 ABC transporter ATPase Antitoxin
BE949_RS27720 23854..24012 - 159 WP_032142560.1 hypothetical protein -
BE949_RS00225 24713..25570 - 858 WP_000130646.1 incFII family plasmid replication initiator RepA -
BE949_RS00230 25563..25637 - 75 WP_001365705.1 RepA leader peptide Tap -
BE949_RS00240 25875..26129 - 255 WP_000083830.1 replication regulatory protein RepA -
BE949_RS00245 26369..26959 - 591 WP_000766807.1 DUF2726 domain-containing protein -
BE949_RS00250 26997..27206 - 210 WP_001298565.1 hemolysin expression modulator Hha -
BE949_RS00255 27252..27713 - 462 WP_001233838.1 thermonuclease family protein -
BE949_RS00260 27958..28170 - 213 WP_001309245.1 ANR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-27 - 1..70152 70152
- flank IS/Tn blaCTX-M-27 - 17779..20166 2387


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10875.53 Da        Isoelectric Point: 8.4615

>T71142 WP_012881118.1 NZ_CP018973:c23533-23252 [Escherichia coli]
MMEIFWTILASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T71142 NZ_CP018973:c23533-23252 [Escherichia coli]
ATGATGGAGATATTCTGGACCATACTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTATATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10206.54 Da        Isoelectric Point: 8.8735

>AT71142 WP_012881119.1 NZ_CP018973:c23799-23530 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSATAWRD
EISRKVAGK

Download         Length: 270 bp

>AT71142 NZ_CP018973:c23799-23530 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACTTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGATATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATAAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Y1YQH8

Antitoxin

Source ID Structure
AlphaFold DB A0A4Y1YSD8

References