Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3779673..3780291 Replicon chromosome
Accession NZ_CP018948
Organism Escherichia coli strain Ecol_224

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BE957_RS22050 Protein ID WP_001291435.1
Coordinates 3780073..3780291 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BE957_RS22045 Protein ID WP_000344800.1
Coordinates 3779673..3780047 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE957_RS22035 3774762..3775955 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BE957_RS22040 3775978..3779127 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
BE957_RS22045 3779673..3780047 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BE957_RS22050 3780073..3780291 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BE957_RS22055 3780462..3781013 + 552 WP_000102564.1 maltose O-acetyltransferase -
BE957_RS22060 3781129..3781599 + 471 WP_000136192.1 YlaC family protein -
BE957_RS22065 3781763..3783313 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BE957_RS22070 3783355..3783708 - 354 WP_000878141.1 DUF1428 family protein -
BE957_RS22080 3784087..3784398 + 312 WP_000409911.1 MGMT family protein -
BE957_RS22085 3784429..3785001 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T70990 WP_001291435.1 NZ_CP018948:3780073-3780291 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T70990 NZ_CP018948:3780073-3780291 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT70990 WP_000344800.1 NZ_CP018948:3779673-3780047 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT70990 NZ_CP018948:3779673-3780047 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References