Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 4591497..4592472 Replicon chromosome
Accession NZ_CP018935
Organism Bacillus cereus strain JEM-2

Toxin (Protein)


Gene name spoIISA Uniprot ID C3GJ28
Locus tag BEI53_RS24920 Protein ID WP_003297560.1
Coordinates 4591735..4592472 (-) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag BEI53_RS24915 Protein ID WP_000588712.1
Coordinates 4591497..4591622 (-) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEI53_RS24875 4587290..4587760 + 471 WP_029438359.1 DUF5065 family protein -
BEI53_RS24885 4588287..4589756 - 1470 WP_000287522.1 beta-Ala-His dipeptidase -
BEI53_RS29660 4589900..4590076 - 177 WP_000808046.1 hypothetical protein -
BEI53_RS24900 4590415..4590651 + 237 WP_001021688.1 hypothetical protein -
BEI53_RS24905 4590928..4591209 + 282 WP_001041554.1 hypothetical protein -
BEI53_RS24915 4591497..4591622 - 126 WP_000588712.1 hypothetical protein Antitoxin
BEI53_RS24920 4591735..4592472 - 738 WP_003297560.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
BEI53_RS24930 4592661..4593398 - 738 WP_000594156.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
BEI53_RS24935 4593566..4594048 - 483 WP_000191912.1 MarR family winged helix-turn-helix transcriptional regulator -
BEI53_RS24940 4594155..4595840 + 1686 WP_003304987.1 alpha-keto acid decarboxylase family protein -
BEI53_RS29665 4595962..4596054 - 93 Protein_4601 methyltransferase -
BEI53_RS24945 4596160..4596567 - 408 WP_000072480.1 VOC family protein -
BEI53_RS24950 4596687..4597347 - 661 Protein_4603 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(9-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28373.07 Da        Isoelectric Point: 8.2672

>T70958 WP_003297560.1 NZ_CP018935:c4592472-4591735 [Bacillus cereus]
VISNIRIGLFILAIVFLILVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T70958 NZ_CP018935:c4592472-4591735 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAATACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT70958 WP_000588712.1 NZ_CP018935:c4591622-4591497 [Bacillus cereus]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT70958 NZ_CP018935:c4591622-4591497 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3R9EK80

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References