Detailed information of TA system TA070843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3955374..3955969
• Replicon: chromosome
• Accession: NZ_CP018917
• Organism: Serratia marcescens strain UMH5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: BVG97_RS19075
• Protein ID: WP_071605322.1
• Coordinates: 3955715..3955969 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BVG97_RS19070
• Protein ID: WP_063988754.1
• Coordinates: 3955374..3955715 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVG97_RS19050	3950882..3951697	-	816	WP_063988643.1	GPO family capsid scaffolding protein	-
BVG97_RS19055	3951840..3953612	+	1773	WP_063988642.1	terminase ATPase subunit family protein	-
BVG97_RS19060	3953612..3954298	+	687	WP_145958035.1	terminase family protein	-
BVG97_RS19065	3954295..3955329	+	1035	WP_033632040.1	phage portal protein	-
BVG97_RS19070	3955374..3955715	-	342	WP_063988754.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BVG97_RS19075	3955715..3955969	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
BVG97_RS19080	3956339..3956536	-	198	WP_126186641.1	hypothetical protein	-
BVG97_RS19085	3956584..3956808	-	225	WP_060445968.1	hypothetical protein	-
BVG97_RS19090	3956847..3959063	-	2217	WP_063988641.1	replication endonuclease	-
BVG97_RS19095	3959060..3959341	-	282	WP_048321968.1	DUF5405 family protein	-
BVG97_RS19100	3959464..3959688	-	225	WP_060426813.1	TraR/DksA C4-type zinc finger protein	-
BVG97_RS19105	3959688..3959999	-	312	WP_060437236.1	DUF5405 family protein	-
BVG97_RS19110	3959999..3960292	-	294	WP_060437235.1	DUF2732 family protein	-
BVG97_RS19115	3960357..3960659	-	303	WP_043148063.1	hypothetical protein	-
BVG97_RS19120	3960671..3960850	-	180	WP_049279101.1	DUF2724 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	aac(6')-Ic	rfaE	3856605..3969735	113130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T70843 WP_071605322.1 NZ_CP018917:c3955969-3955715 [Serratia marcescens]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T70843 NZ_CP018917:c3955969-3955715 [Serratia marcescens]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12390.89 Da
• Isoelectric Point: 4.4537

>AT70843 WP_063988754.1 NZ_CP018917:c3955715-3955374 [Serratia marcescens]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECQKDGIEPYKSYSGKVTTRLSP
ERHQALALAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT70843 NZ_CP018917:c3955715-3955374 [Serratia marcescens]
ATGAATAACACACTGAAAATTGACGGCCATACGGCCGTCATCAACTTCGACCCTGAAATTGAAATGTTCCGGGGCGAGTT
TGTCGGGCTGAACGGCGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGATCTCACTCGCGG
TCTTTCTCGATGAGTGCCAAAAAGACGGCATCGAGCCTTACAAGTCGTACAGCGGCAAAGTAACCACCCGCCTGTCGCCG
GAACGTCATCAGGCGTTAGCCCTTGCCGCACAGGCCACGGGGCAGTCGATTAACGAGCTGCTCAATGAGGGTGTCGATCT
GGTTATCGAAAAGCATTCCTGA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1