Detailed information of TA system TA070755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 108846..109489
• Replicon: plasmid unnamed1
• Accession: NZ_CP018884
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain BR7

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A5Q9LNG2
• Locus tag: BU195_RS27605
• Protein ID: WP_048983057.1
• Coordinates: 109073..109489 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: BU195_RS27600
• Protein ID: WP_001261276.1
• Coordinates: 108846..109076 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BU195_RS27555	104034..104507	+	474	WP_004152341.1	YkgJ family cysteine cluster protein	-
BU195_RS27560	104599..104829	+	231	WP_011977773.1	hypothetical protein	-
BU195_RS27565	105721..106503	-	783	WP_004152340.1	site-specific integrase	-
BU195_RS27570	106503..106835	-	333	WP_004152339.1	hypothetical protein	-
BU195_RS27575	106842..107240	-	399	WP_004171440.1	hypothetical protein	-
BU195_RS27580	107266..107595	-	330	WP_004152337.1	hypothetical protein	-
BU195_RS27585	107623..107931	-	309	WP_004152336.1	hypothetical protein	-
BU195_RS27600	108846..109076	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BU195_RS27605	109073..109489	+	417	WP_048983057.1	type II toxin-antitoxin system VapC family toxin	Toxin
BU195_RS27610	109530..110407	-	878	Protein_124	restriction endonuclease	-
BU195_RS27615	111066..112343	+	1278	WP_016338369.1	HlyD family secretion protein	-
BU195_RS27620	112333..114441	+	2109	WP_016338368.1	peptidase domain-containing ABC transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catA1 / aac(3)-IId / blaTEM-1B / blaCTX-M-15 / blaKPC-2 / qnrB1 / qacE / dfrA30	-	1..121863	121863
	-	flank	IS/Tn	-	-	102646..103914	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15022.52 Da
• Isoelectric Point: 9.2957

>T70755 WP_048983057.1 NZ_CP018884:109073-109489 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T70755 NZ_CP018884:109073-109489 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT70755 WP_001261276.1 NZ_CP018884:108846-109076 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT70755 NZ_CP018884:108846-109076 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LNG2

Antitoxin

• AlphaFold DB: A0A387K023