Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1953210..1953830 Replicon chromosome
Accession NZ_CP018785
Organism Enterobacter ludwigii strain AA4

Toxin (Protein)


Gene name Hha Uniprot ID G8LPY2
Locus tag BUE86_RS09370 Protein ID WP_014168902.1
Coordinates 1953612..1953830 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID W0BRV6
Locus tag BUE86_RS09365 Protein ID WP_020885187.1
Coordinates 1953210..1953584 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BUE86_RS09355 1948336..1949529 + 1194 WP_020885186.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BUE86_RS09360 1949552..1952698 + 3147 WP_014168904.1 multidrug efflux RND transporter permease subunit -
BUE86_RS09365 1953210..1953584 + 375 WP_020885187.1 Hha toxicity modulator TomB Antitoxin
BUE86_RS09370 1953612..1953830 + 219 WP_014168902.1 hemolysin expression modulator Hha Toxin
BUE86_RS09375 1954040..1954591 + 552 WP_020885188.1 maltose O-acetyltransferase -
BUE86_RS09380 1954709..1955176 + 468 WP_079231349.1 hypothetical protein -
BUE86_RS09385 1955148..1956608 - 1461 WP_079231350.1 PLP-dependent aminotransferase family protein -
BUE86_RS09390 1956709..1957419 + 711 WP_079231351.1 GNAT family N-acetyltransferase -
BUE86_RS09395 1957416..1957556 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
BUE86_RS09400 1957559..1957819 - 261 WP_010428154.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8654.04 Da        Isoelectric Point: 8.9107

>T70523 WP_014168902.1 NZ_CP018785:1953612-1953830 [Enterobacter ludwigii]
MSEKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWRFVR

Download         Length: 219 bp

>T70523 NZ_CP018785:1953612-1953830 [Enterobacter ludwigii]
ATGTCTGAAAAACCACTCACAAAAATCGATTATTTGATGCGTTTGCGACGCTGTCAGTCAATAGACACGCTTGAACGTGT
AATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCGGCTGACCACCGTCTTGCAGAAC
TGACTATGAATAAGCTGTATGACAAAATCCCAACGTCCGTGTGGAGATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8886

>AT70523 WP_020885187.1 NZ_CP018785:1953210-1953584 [Enterobacter ludwigii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFINVSRANPVSLSC

Download         Length: 375 bp

>AT70523 NZ_CP018785:1953210-1953584 [Enterobacter ludwigii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTAAACGAGTTGATCGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGATGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAATGCGCAAGATTTGCAGAAATGGCGTAAATCGGGCAACCGACT
ATTTCGTTGCTTCATCAACGTGAGCAGAGCTAACCCCGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A839BM33

Antitoxin

Source ID Structure
AlphaFold DB A0A5C1BX74

References