Detailed information of TA system TA070166

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1989197..1989969
• Replicon: chromosome
• Accession: NZ_CP018671
• Organism: Klebsiella pneumoniae strain CAV1042

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6ICB7
• Locus tag: AGE78_RS12270
• Protein ID: WP_049238634.1
• Coordinates: 1989197..1989586 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AGE78_RS12275
• Protein ID: WP_171547612.1
• Coordinates: 1989643..1989969 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGE78_RS12250	1984316..1985119	+	804	WP_002916291.1	carboxymuconolactone decarboxylase family protein	-
AGE78_RS12255	1985483..1986412	-	930	WP_004185541.1	LysR family transcriptional regulator	-
AGE78_RS12260	1986542..1987930	+	1389	WP_032425235.1	MFS transporter	-
AGE78_RS12265	1988271..1989110	-	840	WP_049238632.1	DUF4942 domain-containing protein	-
AGE78_RS12270	1989197..1989586	-	390	WP_049238634.1	TA system toxin CbtA family protein	Toxin
AGE78_RS12275	1989643..1989969	-	327	WP_171547612.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AGE78_RS12280	1990027..1990248	-	222	WP_049238638.1	DUF987 domain-containing protein	-
AGE78_RS12285	1990262..1990741	-	480	WP_049238640.1	DNA repair protein RadC	-
AGE78_RS12290	1990753..1991196	-	444	WP_049238643.1	antirestriction protein	-
AGE78_RS12295	1991276..1991533	-	258	WP_049238645.1	DUF905 domain-containing protein	-
AGE78_RS12300	1991530..1992354	-	825	WP_049238647.1	DUF945 domain-containing protein	-
AGE78_RS12310	1993003..1993635	+	633	WP_049238458.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14387.45 Da
• Isoelectric Point: 9.6254

>T70166 WP_049238634.1 NZ_CP018671:c1989586-1989197 [Klebsiella pneumoniae]
MQTKLSPLMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDEQVIQQHIDAGISLSDALNFIVEKYELVRTDRPGF
SIREQSPFITPIDILRARKAAGLMNRGTYKEVTAITRGQHPQANASGKR

Nucleotide

• Length: 390 bp

>T70166 NZ_CP018671:c1989586-1989197 [Klebsiella pneumoniae]
ATGCAAACCAAATTATCCCCCCTCATGCGGGCGGCATCGTCACGCCCGTCTCCCGTGGGTGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACATCACTATGGTCTCACGCTTAACGATACGCCGTTCAGTGATGAACAGGTGATTCAGCAGCATATCG
ACGCAGGTATCTCGCTGTCCGATGCCCTGAATTTTATTGTAGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATCACACCCATTGATATCCTCCGGGCCAGAAAGGCTGCGGGGCTGATGAACCGTGG
CACGTATAAAGAGGTGACGGCTATTACCAGAGGACAACATCCGCAGGCCAACGCTTCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12174.84 Da
• Isoelectric Point: 6.4673

>AT70166 WP_171547612.1 NZ_CP018671:c1989969-1989643 [Klebsiella pneumoniae]
ISEPWWGLRRSVSPCFGARLVQERNSLHYLADRANFDGQFCDADLRYLDQAFPVLMKQLELMLTSGELNPRHQHGVTLYA
KGLTCEADTLGSHGYVYIAIYPTPVTTA

Nucleotide

• Length: 327 bp

>AT70166 NZ_CP018671:c1989969-1989643 [Klebsiella pneumoniae]
ATCAGCGAACCCTGGTGGGGGCTCAGACGCAGTGTCTCCCCGTGTTTTGGGGCCCGTCTGGTCCAGGAGAGAAACTCCCT
GCATTACCTGGCTGACCGTGCGAACTTTGATGGTCAGTTCTGCGATGCGGATTTGCGCTATCTTGACCAGGCCTTCCCGG
TACTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAATCCCCGTCATCAGCACGGCGTCACACTGTATGCA
AAAGGTCTGACCTGCGAGGCGGATACGCTCGGTTCGCACGGCTACGTTTATATCGCGATTTATCCCACTCCCGTCACCAC
GGCATAA

Structures

Toxin

• AlphaFold DB: A0A7L6ICB7