Detailed information of TA system TA070153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 767..1524
• Replicon: plasmid pCAV1042-183
• Accession: NZ_CP018670
• Organism: Klebsiella pneumoniae strain CAV1042

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J4ZXV7
• Locus tag: AGE78_RS00910
• Protein ID: WP_029497408.1
• Coordinates: 767..1249 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: AGE78_RS00915
• Protein ID: WP_094645258.1
• Coordinates: 1237..1524 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGE78_RS00910	767..1249	-	483	WP_029497408.1	GNAT family N-acetyltransferase	Toxin
AGE78_RS00915	1237..1524	-	288	WP_094645258.1	DUF1778 domain-containing protein	Antitoxin
AGE78_RS00920	1749..2114	+	366	WP_074075300.1	hypothetical protein	-
AGE78_RS00925	2158..2895	+	738	WP_008460272.1	HupE/UreJ family protein	-
AGE78_RS00930	2909..3598	+	690	WP_004196322.1	hypothetical protein	-
AGE78_RS00935	3629..5005	-	1377	WP_004196363.1	chromate efflux transporter	-
AGE78_RS00940	4962..5939	-	978	WP_004196334.1	chromate resistance protein	-
AGE78_RS30705	5969..6161	+	193	Protein_8	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..183432	183432
	-	flank	IS/Tn	-	-	20..370	350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17599.34 Da
• Isoelectric Point: 9.8719

>T70153 WP_029497408.1 NZ_CP018670:c1249-767 [Klebsiella pneumoniae]
VGRLTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLRLP

Nucleotide

• Length: 483 bp

>T70153 NZ_CP018670:c1249-767 [Klebsiella pneumoniae]
GTGGGACGTTTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTGGCTGAGTTCGTCAGCGGTGAGGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCCCTCGGAGCTGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGCGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAGCGAACATTGTTTCTTAGACTCCCT
TAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10955.66 Da
• Isoelectric Point: 4.5141

>AT70153 WP_094645258.1 NZ_CP018670:c1524-1237 [Klebsiella pneumoniae]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVVDD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT70153 NZ_CP018670:c1524-1237 [Klebsiella pneumoniae]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGCGGAGTTCATCGACATGCTTGATGCACCTGTCGTAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2J4ZXV7