Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3534357..3535027 Replicon chromosome
Accession NZ_CP018303
Organism Mycobacterium tuberculosis strain I0004241-1

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag BOJ25_RS17350 Protein ID WP_003899954.1
Coordinates 3534357..3534701 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag BOJ25_RS17355 Protein ID WP_003899955.1
Coordinates 3534698..3535027 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ25_RS17315 3529430..3530290 + 861 WP_003416628.1 alpha/beta hydrolase -
BOJ25_RS17320 3530265..3530780 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
BOJ25_RS17330 3531020..3531313 + 294 WP_003416635.1 hypothetical protein -
BOJ25_RS17335 3531601..3532890 + 1290 WP_070171107.1 ATP-binding protein -
BOJ25_RS17340 3533237..3533671 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
BOJ25_RS17345 3533674..3534126 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BOJ25_RS17350 3534357..3534701 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BOJ25_RS17355 3534698..3535027 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
BOJ25_RS17360 3535565..3535912 + 348 WP_003899956.1 DUF2384 domain-containing protein -
BOJ25_RS17365 3535909..3536529 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
BOJ25_RS17370 3536689..3537954 - 1266 WP_003902423.1 hypothetical protein -
BOJ25_RS17375 3538122..3538331 + 210 WP_003416778.1 hypothetical protein -
BOJ25_RS17385 3538578..3539612 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T69541 WP_003899954.1 NZ_CP018303:3534357-3534701 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T69541 NZ_CP018303:3534357-3534701 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT69541 WP_003899955.1 NZ_CP018303:3534698-3535027 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT69541 NZ_CP018303:3534698-3535027 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References