Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3093408..3093978 Replicon chromosome
Accession NZ_CP018303
Organism Mycobacterium tuberculosis strain I0004241-1

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag BOJ25_RS15220 Protein ID WP_003414166.1
Coordinates 3093408..3093764 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag BOJ25_RS15225 Protein ID WP_003901465.1
Coordinates 3093748..3093978 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ25_RS15200 3088860..3090548 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
BOJ25_RS15205 3090552..3090878 - 327 WP_003414157.1 hypothetical protein -
BOJ25_RS15210 3091051..3091638 + 588 WP_003914429.1 DUF3558 family protein -
BOJ25_RS15215 3091657..3093306 + 1650 WP_070171102.1 CocE/NonD family hydrolase -
BOJ25_RS15220 3093408..3093764 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
BOJ25_RS15225 3093748..3093978 - 231 WP_003901465.1 antitoxin MazE Antitoxin
BOJ25_RS15230 3094021..3095064 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
BOJ25_RS21685 3095255..3095530 + 276 WP_003911993.1 DUF1778 domain-containing protein -
BOJ25_RS21690 3095706..3095960 - 255 WP_003917684.1 hypothetical protein -
BOJ25_RS15250 3096108..3096512 + 405 WP_009938577.1 hypothetical protein -
BOJ25_RS15255 3096509..3096700 + 192 WP_003414184.1 hypothetical protein -
BOJ25_RS15260 3096764..3098053 + 1290 Protein_2885 transposase family protein -
BOJ25_RS15265 3098287..3098544 + 258 WP_003899489.1 hypothetical protein -
BOJ25_RS15270 3098649..3098960 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T69536 WP_003414166.1 NZ_CP018303:c3093764-3093408 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T69536 NZ_CP018303:c3093764-3093408 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT69536 WP_003901465.1 NZ_CP018303:c3093978-3093748 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT69536 NZ_CP018303:c3093978-3093748 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References