Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 72966..73525 Replicon plasmid pO157
Accession NZ_CP018248
Organism Escherichia coli strain 7784

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag BFL18_RS29720 Protein ID WP_000421248.1
Coordinates 73250..73525 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag BFL18_RS29715 Protein ID WP_001178089.1
Coordinates 72966..73250 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL18_RS29660 68157..68717 + 561 WP_000840472.1 fertility inhibition protein FinO -
BFL18_RS29665 68850..69062 + 213 WP_001302189.1 ANR family transcriptional regulator -
BFL18_RS29670 69307..69768 + 462 WP_001233853.1 thermonuclease family protein -
BFL18_RS29675 69814..70023 + 210 WP_001302200.1 hemolysin expression modulator Hha -
BFL18_RS29680 70061..70399 + 339 WP_000766796.1 hypothetical protein -
BFL18_RS29685 70639..70893 + 255 WP_000083831.1 replication regulatory protein RepA -
BFL18_RS29695 71129..71203 + 75 WP_001370046.1 RepA leader peptide Tap -
BFL18_RS29700 71196..72053 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
BFL18_RS29715 72966..73250 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
BFL18_RS29720 73250..73525 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BFL18_RS29725 73620..73826 + 207 WP_001105064.1 hypothetical protein -
BFL18_RS29735 73926..74141 - 216 Protein_79 transposase -
BFL18_RS29740 74185..74751 - 567 Protein_80 IS3-like element IS600 family transposase -
BFL18_RS29745 74751..75167 - 417 Protein_81 IS91 family transposase -
BFL18_RS29750 75167..75352 - 186 WP_001302179.1 hypothetical protein -
BFL18_RS29755 75529..77739 + 2211 WP_000592771.1 catalase/peroxidase KatP -
BFL18_RS29760 77783..78172 + 390 WP_001172748.1 cytochrome b562 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP 1..91353 91353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T69208 WP_000421248.1 NZ_CP018248:73250-73525 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T69208 NZ_CP018248:73250-73525 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT69208 WP_001178089.1 NZ_CP018248:72966-73250 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT69208 NZ_CP018248:72966-73250 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References