Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 550018..550636 Replicon chromosome
Accession NZ_CP018239
Organism Escherichia coli strain 272

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BFL22_RS02795 Protein ID WP_001291435.1
Coordinates 550018..550236 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BFL22_RS02800 Protein ID WP_000344800.1
Coordinates 550262..550636 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL22_RS02760 545305..545877 + 573 WP_000779831.1 YbaY family lipoprotein -
BFL22_RS02765 545910..546221 - 312 WP_000409911.1 MGMT family protein -
BFL22_RS02775 546600..546953 + 354 WP_000878140.1 DUF1428 domain-containing protein -
BFL22_RS02780 546995..548545 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BFL22_RS02785 548709..549179 - 471 WP_000136192.1 YlaC family protein -
BFL22_RS02790 549295..549846 - 552 WP_000102553.1 maltose O-acetyltransferase -
BFL22_RS02795 550018..550236 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BFL22_RS02800 550262..550636 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BFL22_RS02805 551182..554331 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
BFL22_RS02810 554354..555547 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T69014 WP_001291435.1 NZ_CP018239:c550236-550018 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T69014 NZ_CP018239:c550236-550018 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT69014 WP_000344800.1 NZ_CP018239:c550636-550262 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT69014 NZ_CP018239:c550636-550262 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References