Detailed information of TA system TA068954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 87301..87832
• Replicon: plasmid pHSL4
• Accession: NZ_CP018225
• Organism: Tardibacter chloracetimidivorans strain JJ-A5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T0GNF5
• Locus tag: BSL82_RS19290
• Protein ID: WP_004212690.1
• Coordinates: 87551..87832 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: T0G968
• Locus tag: BSL82_RS19285
• Protein ID: WP_004212689.1
• Coordinates: 87301..87564 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSL82_RS19265	82476..84182	+	1707	WP_062343367.1	copper resistance system multicopper oxidase	-
BSL82_RS19270	84179..85165	+	987	WP_004212678.1	copper resistance protein B	-
BSL82_RS19275	85159..86400	+	1242	WP_007683345.1	MFS transporter	-
BSL82_RS19280	86480..86917	+	438	WP_007683347.1	metal-binding protein	-
BSL82_RS19285	87301..87564	+	264	WP_004212689.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BSL82_RS19290	87551..87832	+	282	WP_004212690.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BSL82_RS20825	88153..88236	+	84	Protein_83	magnesium transporter	-
BSL82_RS19300	88234..88928	+	695	Protein_84	IS21 family transposase	-
BSL82_RS19305	88915..89643	+	729	WP_025549137.1	IS21-like element helper ATPase IstB	-
BSL82_RS19310	89815..90273	+	459	WP_162274424.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..182640	182640
	-	flank	IS/Tn	-	-	88915..89643	728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10993.49 Da
• Isoelectric Point: 7.4752

>T68954 WP_004212690.1 NZ_CP018225:87551-87832 [Tardibacter chloracetimidivorans]
MRTIERFGRFKRDYKREKKGQHAKTLDADLIPIIEALASDEPLEPRHRDHALTGDWRDHRDCHIKPDLVLIYRKPDDDTL
QLVRLGSHAELGW

Nucleotide

• Length: 282 bp

>T68954 NZ_CP018225:87551-87832 [Tardibacter chloracetimidivorans]
ATGCGGACGATTGAACGCTTCGGGCGGTTCAAGCGCGACTACAAGCGGGAGAAGAAGGGGCAGCACGCCAAGACGCTGGA
CGCTGACCTGATCCCGATCATCGAGGCGCTGGCATCCGACGAGCCGCTTGAACCGCGCCACCGCGACCATGCGCTGACCG
GCGACTGGCGCGACCATCGCGATTGCCACATAAAGCCCGACCTCGTGCTGATTTACCGCAAGCCCGATGACGACACCTTG
CAGCTTGTGCGCTTGGGATCGCACGCGGAATTGGGTTGGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9284.75 Da
• Isoelectric Point: 4.5881

>AT68954 WP_004212689.1 NZ_CP018225:87301-87564 [Tardibacter chloracetimidivorans]
MAANALVQTRIDGAVKEEAATVLAAMGLTVSDAVRLMLTRVARDKALPFEPLVPNAETIEAMKEAREGKGKRFATVVDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT68954 NZ_CP018225:87301-87564 [Tardibacter chloracetimidivorans]
ATGGCCGCAAACGCTCTTGTGCAGACCCGGATCGACGGAGCGGTGAAGGAGGAAGCCGCGACCGTGCTGGCTGCTATGGG
GCTGACCGTCTCCGATGCCGTGCGCCTGATGCTGACCCGCGTTGCCCGCGACAAGGCCTTGCCGTTTGAACCGCTGGTGC
CCAATGCCGAGACGATCGAGGCCATGAAGGAAGCGCGCGAGGGAAAGGGCAAGCGGTTCGCCACGGTGGTCGACCTGATG
GCCGACCTCAATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: T0GNF5

Antitoxin

• AlphaFold DB: T0G968