Detailed information of TA system TA068521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1417305..1417960
• Replicon: chromosome
• Accession: NZ_CP018005
• Organism: Coxiella burnetii strain RSA439

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q83BL3
• Locus tag: BMW92_RS07685
• Protein ID: WP_010958264.1
• Coordinates: 1417622..1417960 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BMW92_RS07680
• Protein ID: WP_010958263.1
• Coordinates: 1417305..1417616 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMW92_RS07655	1412583..1413260	-	678	WP_010958258.1	cyclin	-
BMW92_RS07665	1413578..1414960	-	1383	WP_010958260.1	cysteine--tRNA ligase	-
BMW92_RS07670	1414951..1416348	-	1398	WP_010958261.1	glutamate--tRNA ligase	-
BMW92_RS07675	1416505..1417236	+	732	WP_010958262.1	UDP-2,3-diacylglucosamine diphosphatase	-
BMW92_RS07680	1417305..1417616	-	312	WP_010958263.1	HigA family addiction module antidote protein	Antitoxin
BMW92_RS07685	1417622..1417960	-	339	WP_010958264.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BMW92_RS07690	1418319..1419464	+	1146	WP_010957722.1	ISAs1 family transposase	-
BMW92_RS07695	1419792..1421465	+	1674	WP_010958265.1	CBU_1493 family Dot/Icm T4SS effector	-
BMW92_RS07700	1421639..1422394	-	756	WP_010958266.1	pyridoxine 5'-phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1418319..1419464	1145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-72)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13409.66 Da
• Isoelectric Point: 10.4588

>T68521 WP_010958264.1 NZ_CP018005:c1417960-1417622 [Coxiella burnetii]
MPLTLYIMLDVTRKTVILEVIIKSFKDKYTKYLYKGVSVSKWQAIRKQAERRLQILDSVTSLDDLRSLPSNRFESLRGNR
KGQFSIRINKQWRICFKWINNEPTEVEIVDYH

Nucleotide

• Length: 339 bp

>T68521 NZ_CP018005:c1417960-1417622 [Coxiella burnetii]
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATAATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATAAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11458.39 Da
• Isoelectric Point: 10.1699

>AT68521 WP_010958263.1 NZ_CP018005:c1417616-1417305 [Coxiella burnetii]
MKMAANRMRPIHPGEILAEELGFLDKMSANQLAKHLAIPTNRVTAILNGARSITADTALRLAKFFGTTPEFWLNLQDAYD
IKMALKKSGKKIEKEVTPYDQAA

Nucleotide

• Length: 312 bp

>AT68521 NZ_CP018005:c1417616-1417305 [Coxiella burnetii]
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG

Structures

Toxin

• AlphaFold DB: Q83BL3