Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 14916..15441 Replicon plasmid pCAV1016-90
Accession NZ_CP017935
Organism Klebsiella pneumoniae strain CAV1016

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag AGE75_RS26815 Protein ID WP_004197633.1
Coordinates 15136..15441 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag AGE75_RS26810 Protein ID WP_006788213.1
Coordinates 14916..15134 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGE75_RS26785 10206..10500 + 295 Protein_14 korC -
AGE75_RS26795 11089..12090 + 1002 WP_009483873.1 DUF4238 domain-containing protein -
AGE75_RS26800 12275..12619 - 345 WP_009483875.1 hypothetical protein -
AGE75_RS29295 13106..13396 - 291 WP_023287115.1 hypothetical protein -
AGE75_RS26805 13440..14363 - 924 WP_009483877.1 hypothetical protein -
AGE75_RS29300 14519..14746 + 228 Protein_19 hypothetical protein -
AGE75_RS26810 14916..15134 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AGE75_RS26815 15136..15441 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
AGE75_RS29980 15499..15810 + 312 WP_023287114.1 hypothetical protein -
AGE75_RS26825 15860..16195 + 336 WP_009309920.1 hypothetical protein -
AGE75_RS26830 16229..17245 + 1017 WP_009483880.1 hypothetical protein -
AGE75_RS30515 17289..17450 + 162 WP_009483881.1 hypothetical protein -
AGE75_RS26835 17443..18222 + 780 WP_023287113.1 site-specific integrase -
AGE75_RS26840 18280..18537 - 258 WP_023287112.1 hypothetical protein -
AGE75_RS30520 18666..18779 - 114 WP_014343462.1 hypothetical protein -
AGE75_RS26850 19315..20181 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA1 / ant(3'')-Ia / qacE / sul1 - 1..89621 89621


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T68383 WP_004197633.1 NZ_CP017935:15136-15441 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T68383 NZ_CP017935:15136-15441 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT68383 WP_006788213.1 NZ_CP017935:14916-15134 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT68383 NZ_CP017935:14916-15134 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References