Detailed information of TA system TA068281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 421908..422541
• Replicon: chromosome
• Accession: NZ_CP017917
• Organism: Vibrio alginolyticus strain K08M4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A347UQS4
• Locus tag: K08M4_RS16795
• Protein ID: WP_012600340.1
• Coordinates: 421908..422240 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K08M4_RS16800
• Protein ID: WP_086050720.1
• Coordinates: 422227..422541 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K08M4_RS16760	417487..418461	+	975	WP_086050717.1	heme o synthase	-
K08M4_RS16765	418644..418997	+	354	WP_009845655.1	hypothetical protein	-
K08M4_RS16770	419094..419765	+	672	WP_017097015.1	DUF3313 domain-containing protein	-
K08M4_RS16775	419778..419996	+	219	WP_009845653.1	DUF3820 family protein	-
K08M4_RS16790	420972..421418	+	447	Protein_344	GNAT family N-acetyltransferase	-
K08M4_RS16795	421908..422240	+	333	WP_012600340.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K08M4_RS16800	422227..422541	+	315	WP_086050720.1	DNA-binding transcriptional regulator	Antitoxin
K08M4_RS16805	422879..423064	-	186	Protein_347	IS110 family transposase	-
K08M4_RS16810	423235..423888	+	654	WP_157665757.1	hypothetical protein	-
K08M4_RS16820	424982..425305	-	324	WP_016786991.1	hypothetical protein	-
K08M4_RS16825	425783..426001	-	219	Protein_350	IS110 family transposase	-
K08M4_RS16830	426102..426623	+	522	WP_086050724.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-104)

Antitoxin

• Antitoxin MqsA/HigA-2 (25-101)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12906.76 Da
• Isoelectric Point: 10.0960

>T68281 WP_012600340.1 NZ_CP017917:421908-422240 [Vibrio alginolyticus]
MKSVFVESTIFEKYRNEYLSDDEFRLFQAELMSNPKQGDVIQGTGGLRKIRVASKGKGKRGGSRVIYYFLDEKRRFYLLT
IYGKNEMSDLTADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T68281 NZ_CP017917:421908-422240 [Vibrio alginolyticus]
ATGAAAAGTGTATTTGTTGAATCGACCATATTTGAAAAGTACCGTAATGAATATCTCAGCGATGATGAGTTTAGGCTTTT
TCAAGCTGAGTTAATGTCTAACCCGAAACAGGGGGACGTGATTCAAGGTACAGGTGGTTTGCGAAAGATTCGAGTTGCAA
GTAAAGGTAAGGGCAAGCGAGGCGGCTCTCGTGTTATCTATTACTTCCTCGATGAAAAGCGTCGCTTCTACTTGCTGACA
ATTTATGGCAAGAATGAAATGTCTGATTTAACTGCAGATCAAAAGAAACAATTAAAGGCTTTTATGGAGGCGTGGCGTAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11704.17 Da
• Isoelectric Point: 6.3290

>AT68281 WP_086050720.1 NZ_CP017917:422227-422541 [Vibrio alginolyticus]
MSNRDLFAELSSALVEAKEHSEGKLTLKTHQVNDISELDISPNEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT68281 NZ_CP017917:422227-422541 [Vibrio alginolyticus]
ATGAGCAATCGTGATCTATTTGCAGAGTTAAGTTCTGCTCTTGTTGAGGCTAAAGAGCATTCAGAGGGTAAACTCACTCT
TAAAACTCATCAAGTTAACGATATCAGTGAGTTAGATATTTCACCTAATGAGATCGTGAGTATCCGTGAACAGTTTAATA
TGTCGCGTGGTGTGTTTGCGCGTTTACTGCATACATCATCCCGAACATTAGAAAACTGGGAACAAGGGCGTAGCGCACCA
AATGGACAAGCTGTGACCCTTCTAAAATTGGTTCAGCGTCATCCGGAAACTCTTTCGCACATCGCTGAGCTATAA

Structures

Toxin

• AlphaFold DB: A0A347UQS4