Detailed information of TA system TA068166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2309372..2310003
• Replicon: chromosome
• Accession: NZ_CP017802
• Organism: Raoultella ornithinolytica strain MG isolate MG01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1Q8YTV3
• Locus tag: BK817_RS10970
• Protein ID: WP_012542177.1
• Coordinates: 2309372..2309548 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2X1QFN9
• Locus tag: BK817_RS10975
• Protein ID: WP_012542176.1
• Coordinates: 2309596..2310003 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BK817_RS10935	2304391..2304630	+	240	WP_071531363.1	hypothetical protein	-
BK817_RS10940	2304671..2305063	+	393	WP_025861428.1	DNA-binding protein	-
BK817_RS10950	2305263..2306294	+	1032	WP_071359307.1	DUF968 domain-containing protein	-
BK817_RS10955	2306307..2306651	+	345	WP_032435861.1	antitermination protein	-
BK817_RS10960	2306646..2307872	-	1227	WP_023328102.1	hypothetical protein	-
BK817_RS10965	2307862..2309007	-	1146	WP_023328101.1	nucleoid-associated protein	-
BK817_RS10970	2309372..2309548	+	177	WP_012542177.1	type II toxin-antitoxin system HicA family toxin	Toxin
BK817_RS10975	2309596..2310003	+	408	WP_012542176.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BK817_RS10980	2310159..2310395	+	237	WP_004111739.1	class II holin family protein	-
BK817_RS10985	2310373..2310903	+	531	WP_023328100.1	lysozyme	-
BK817_RS10990	2310936..2311412	+	477	WP_050583645.1	DUF2514 domain-containing protein	-
BK817_RS26300	2311494..2311655	+	162	WP_023328098.1	hypothetical protein	-
BK817_RS10995	2311639..2312001	+	363	WP_081351909.1	HNH endonuclease	-
BK817_RS11000	2311953..2312270	+	318	WP_016160653.1	hypothetical protein	-
BK817_RS11005	2312267..2312698	+	432	WP_081351910.1	hypothetical protein	-
BK817_RS11010	2312948..2313382	+	435	WP_012542168.1	phage terminase small subunit P27 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2287975..2342131	54156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6632.83 Da
• Isoelectric Point: 11.0174

>T68166 WP_012542177.1 NZ_CP017802:2309372-2309548 [Raoultella ornithinolytica]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Nucleotide

• Length: 177 bp

>T68166 NZ_CP017802:2309372-2309548 [Raoultella ornithinolytica]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AGCTCGGCATCAAATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14977.99 Da
• Isoelectric Point: 4.4277

>AT68166 WP_012542176.1 NZ_CP017802:2309596-2310003 [Raoultella ornithinolytica]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARMINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Nucleotide

• Length: 408 bp

>AT68166 NZ_CP017802:2309596-2310003 [Raoultella ornithinolytica]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACAGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCTTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCATGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTATCTCTTGGTCACAGCACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Structures

Toxin

• AlphaFold DB: A0A1Q8YTV3

Antitoxin

• AlphaFold DB: A0A2X1QFN9