Detailed information of TA system TA068061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3817882..3818456
• Replicon: chromosome
• Accession: NZ_CP017689
• Organism: Thalassotalea crassostreae strain LPB0090

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LT090_RS16550
• Protein ID: WP_068547893.1
• Coordinates: 3818178..3818456 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LT090_RS16545
• Protein ID: WP_068547894.1
• Coordinates: 3817882..3818178 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT090_RS16510	3813306..3813650	-	345	WP_068547702.1	hypothetical protein	-
LT090_RS16515	3813758..3814051	-	294	WP_068547701.1	HigA family addiction module antidote protein	-
LT090_RS16520	3814051..3814329	-	279	WP_068547700.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LT090_RS16525	3814542..3815585	+	1044	WP_068547699.1	IS110 family transposase	-
LT090_RS17040	3815829..3815972	+	144	WP_157726661.1	hypothetical protein	-
LT090_RS16530	3816047..3816493	-	447	WP_157726666.1	hypothetical protein	-
LT090_RS16535	3816590..3817219	-	630	WP_068547896.1	PEP-CTERM sorting domain-containing protein	-
LT090_RS16540	3817513..3817758	-	246	WP_068547895.1	hypothetical protein	-
LT090_RS16545	3817882..3818178	-	297	WP_068547894.1	HigA family addiction module antidote protein	Antitoxin
LT090_RS16550	3818178..3818456	-	279	WP_068547893.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LT090_RS16555	3818624..3819337	-	714	WP_068547892.1	subclass B1 metallo-beta-lactamase	-
LT090_RS16560	3819454..3819768	-	315	WP_068547891.1	excalibur calcium-binding domain-containing protein	-
LT090_RS16565	3819887..3820150	-	264	WP_157726665.1	hypothetical protein	-
LT090_RS16570	3820234..3821166	-	933	WP_068547889.1	hypothetical protein	-
LT090_RS16575	3821511..3821732	-	222	WP_068547888.1	hypothetical protein	-
LT090_RS16580	3821923..3822966	+	1044	WP_068547699.1	IS110 family transposase	-
LT090_RS17045	3823210..3823353	+	144	WP_157726661.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3814542..3815585	1043
	-	inside	Integron	-	-	3813664..3829303	15639

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10835.47 Da
• Isoelectric Point: 9.9958

>T68061 WP_068547893.1 NZ_CP017689:c3818456-3818178 [Thalassotalea crassostreae]
MIKSFIHKGLRKFYSSGSTVGIQKKHEKKLRLILANLDQAEEPDDMDLPGLFMHQLKGDRKGVWSVRVNGNWRITYRFIG
RDVEIVNYEDYH

Nucleotide

• Length: 279 bp

>T68061 NZ_CP017689:c3818456-3818178 [Thalassotalea crassostreae]
ATGATTAAAAGTTTCATTCATAAAGGTCTTCGAAAGTTCTACTCTTCAGGTAGTACTGTAGGAATCCAGAAAAAGCACGA
AAAGAAACTACGTCTAATTCTTGCTAACTTAGATCAAGCAGAAGAACCAGACGATATGGATCTACCTGGTTTATTTATGC
ATCAACTTAAAGGTGACAGGAAAGGCGTATGGTCAGTTCGAGTAAACGGGAATTGGCGCATAACGTATAGATTTATTGGA
CGTGACGTTGAAATTGTAAATTATGAGGATTACCACTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11045.70 Da
• Isoelectric Point: 7.4699

>AT68061 WP_068547894.1 NZ_CP017689:c3818178-3817882 [Thalassotalea crassostreae]
MAMFNPAHPGEILKELVIDSLELTITDVAKHLDVSRKTLSKVLNAKGSVTPEMAVRLELAFGKPSADHWLRLQNAHDLWR
TRQEQSSLHVSPYNFQVA

Nucleotide

• Length: 297 bp

>AT68061 NZ_CP017689:c3818178-3817882 [Thalassotalea crassostreae]
ATGGCTATGTTTAACCCTGCACATCCGGGGGAAATATTAAAAGAGTTAGTGATCGACTCTTTAGAGTTAACGATCACGGA
TGTTGCAAAGCACCTTGATGTAAGTCGAAAAACTTTATCTAAAGTTTTAAATGCAAAAGGGTCTGTAACCCCCGAGATGG
CTGTCCGTCTTGAATTAGCTTTTGGAAAACCATCAGCAGATCATTGGCTTCGTTTACAGAATGCACATGATTTATGGAGA
ACAAGACAAGAGCAAAGCTCATTACATGTTTCACCGTATAATTTTCAAGTGGCATAA