Detailed information of TA system TA067851

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 103985..104411
• Replicon: plasmid pSLK172-2
• Accession: NZ_CP017633
• Organism: Escherichia coli strain SLK172

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BJJ90_RS31595
• Protein ID: WP_001312861.1
• Coordinates: 103985..104143 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	104187..104411 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJJ90_RS31550	99040..99267	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
BJJ90_RS31555	99361..100047	-	687	WP_015059009.1	PAS domain-containing protein	-
BJJ90_RS31560	100238..100621	-	384	WP_000124981.1	relaxosome protein TraM	-
BJJ90_RS31565	100898..101545	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
BJJ90_RS31570	101842..102663	-	822	WP_001234445.1	DUF945 domain-containing protein	-
BJJ90_RS31575	102774..103070	-	297	WP_001272251.1	hypothetical protein	-
BJJ90_RS33960	103370..103747	+	378	Protein_122	hypothetical protein	-
BJJ90_RS31595	103985..104143	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	104187..104411	-	225	NuclAT_0	-	Antitoxin
-	104187..104411	-	225	NuclAT_0	-	Antitoxin
-	104187..104411	-	225	NuclAT_0	-	Antitoxin
-	104187..104411	-	225	NuclAT_0	-	Antitoxin
BJJ90_RS33590	104223..104402	+	180	WP_001309233.1	hypothetical protein	-
BJJ90_RS31605	104423..105142	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
BJJ90_RS31610	105139..105573	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
BJJ90_RS31615	105642..107665	-	2024	Protein_127	ParB/RepB/Spo0J family partition protein	-
BJJ90_RS31620	107726..107959	-	234	WP_000006003.1	DUF905 family protein	-
BJJ90_RS31625	108017..108544	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
BJJ90_RS33965	108846..109295	+	450	WP_045892832.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / fosA3 / oqxA / oqxB / aph(3')-IIa / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..120528	120528

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T67851 WP_001312861.1 NZ_CP017633:c104143-103985 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T67851 NZ_CP017633:c104143-103985 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT67851 NZ_CP017633:c104411-104187 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin