Detailed information of TA system TA067819

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2346246..2346617
• Replicon: chromosome
• Accession: NZ_CP017631
• Organism: Escherichia coli strain SLK172

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: BJJ90_RS12620
• Protein ID: WP_001317028.1
• Coordinates: 2346423..2346617 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2346246..2346424 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJJ90_RS12595	2342201..2343574	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
BJJ90_RS12600	2343703..2344638	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
BJJ90_RS12605	2344690..2345925	-	1236	Protein_2239	site-specific integrase	-
BJJ90_RS12610	2345927..2346142	-	216	WP_000079604.1	excisionase XisR	-
BJJ90_RS12615	2346221..2346430	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2346246..2346424	+	179	NuclAT_0	-	Antitoxin
-	2346246..2346424	+	179	NuclAT_0	-	Antitoxin
-	2346246..2346424	+	179	NuclAT_0	-	Antitoxin
-	2346246..2346424	+	179	NuclAT_0	-	Antitoxin
BJJ90_RS12620	2346423..2346617	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
BJJ90_RS12625	2346674..2347483	-	810	WP_000166319.1	recombination protein RecT	-
BJJ90_RS12630	2347476..2350076	-	2601	WP_075362597.1	exodeoxyribonuclease VIII	-
BJJ90_RS12635	2350178..2350452	-	275	Protein_2245	hypothetical protein	-
BJJ90_RS12640	2350527..2350697	-	171	WP_001352098.1	conserved protein, Rac prophage	-
BJJ90_RS12645	2350697..2350918	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	vgrG/tssI / vgrG/tssI / rhs/PAAR / espR1 / espR1 / sodB	2331608..2698747	367139

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T67819 WP_001317028.1 NZ_CP017631:c2346617-2346423 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T67819 NZ_CP017631:c2346617-2346423 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT67819 NZ_CP017631:2346246-2346424 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin