Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3170216..3170903 Replicon chromosome
Accession NZ_CP017596
Organism Mycobacterium tuberculosis strain Beijing/391

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag BJM02_RS15645 Protein ID WP_003414624.1
Coordinates 3170460..3170903 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag BJM02_RS15640 Protein ID WP_003414620.1
Coordinates 3170216..3170473 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJM02_RS15620 3165536..3166390 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
BJM02_RS15625 3166446..3167609 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
BJM02_RS15630 3167626..3168840 - 1215 WP_003414610.1 zinc metalloprotease Rip -
BJM02_RS15635 3168848..3170089 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
BJM02_RS15640 3170216..3170473 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
BJM02_RS15645 3170460..3170903 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
BJM02_RS15650 3170983..3171645 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
BJM02_RS15655 3171741..3171932 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BJM02_RS15660 3172264..3174012 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
BJM02_RS15665 3174108..3174689 + 582 WP_003414644.1 fasciclin domain-containing protein -
BJM02_RS15670 3174789..3175055 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T67672 WP_003414624.1 NZ_CP017596:3170460-3170903 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T67672 NZ_CP017596:3170460-3170903 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT67672 WP_003414620.1 NZ_CP017596:3170216-3170473 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT67672 NZ_CP017596:3170216-3170473 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References