Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2864278..2864915 Replicon chromosome
Accession NZ_CP017596
Organism Mycobacterium tuberculosis strain Beijing/391

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BJM02_RS13945 Protein ID WP_003413180.1
Coordinates 2864278..2864673 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BJM02_RS13950 Protein ID WP_003413183.1
Coordinates 2864670..2864915 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJM02_RS13900 2859681..2860892 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BJM02_RS13910 2861019..2861678 + 660 WP_003902296.1 hypothetical protein -
BJM02_RS13915 2861675..2862338 + 664 Protein_2628 hypothetical protein -
BJM02_RS21630 2862335..2862612 + 278 Protein_2629 type II toxin-antitoxin system VapB family antitoxin -
BJM02_RS13925 2862705..2863118 + 414 WP_003413164.1 PIN domain nuclease -
BJM02_RS13930 2863157..2863414 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BJM02_RS13935 2863411..2863788 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BJM02_RS13940 2863804..2864178 - 375 WP_003413177.1 hypothetical protein -
BJM02_RS13945 2864278..2864673 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BJM02_RS13950 2864670..2864915 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BJM02_RS13960 2865326..2865745 - 420 WP_003413190.1 A24 family peptidase -
BJM02_RS13965 2865757..2866566 - 810 WP_003413193.1 shikimate dehydrogenase -
BJM02_RS13970 2866563..2867816 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BJM02_RS13975 2867809..2868321 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T67663 WP_003413180.1 NZ_CP017596:c2864673-2864278 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T67663 NZ_CP017596:c2864673-2864278 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT67663 WP_003413183.1 NZ_CP017596:c2864915-2864670 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT67663 NZ_CP017596:c2864915-2864670 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References