Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2863157..2863788 Replicon chromosome
Accession NZ_CP017596
Organism Mycobacterium tuberculosis strain Beijing/391

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag BJM02_RS13935 Protein ID WP_003413174.1
Coordinates 2863411..2863788 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag BJM02_RS13930 Protein ID WP_003413167.1
Coordinates 2863157..2863414 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJM02_RS13895 2859071..2859385 + 315 WP_009937839.1 hypothetical protein -
BJM02_RS13900 2859681..2860892 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BJM02_RS13910 2861019..2861678 + 660 WP_003902296.1 hypothetical protein -
BJM02_RS13915 2861675..2862338 + 664 Protein_2628 hypothetical protein -
BJM02_RS21630 2862335..2862612 + 278 Protein_2629 type II toxin-antitoxin system VapB family antitoxin -
BJM02_RS13925 2862705..2863118 + 414 WP_003413164.1 PIN domain nuclease -
BJM02_RS13930 2863157..2863414 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
BJM02_RS13935 2863411..2863788 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
BJM02_RS13940 2863804..2864178 - 375 WP_003413177.1 hypothetical protein -
BJM02_RS13945 2864278..2864673 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
BJM02_RS13950 2864670..2864915 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
BJM02_RS13960 2865326..2865745 - 420 WP_003413190.1 A24 family peptidase -
BJM02_RS13965 2865757..2866566 - 810 WP_003413193.1 shikimate dehydrogenase -
BJM02_RS13970 2866563..2867816 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BJM02_RS13975 2867809..2868321 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T67662 WP_003413174.1 NZ_CP017596:2863411-2863788 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T67662 NZ_CP017596:2863411-2863788 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT67662 WP_003413167.1 NZ_CP017596:2863157-2863414 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT67662 NZ_CP017596:2863157-2863414 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References