Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2892212..2892849 Replicon chromosome
Accession NZ_CP017594
Organism Mycobacterium tuberculosis strain Beijing-like/36918

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BJM00_RS14070 Protein ID WP_003413180.1
Coordinates 2892212..2892607 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BJM00_RS14075 Protein ID WP_003413183.1
Coordinates 2892604..2892849 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJM00_RS14025 2887615..2888826 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BJM00_RS14035 2888953..2889612 + 660 WP_003902296.1 hypothetical protein -
BJM00_RS14040 2889609..2890272 + 664 Protein_2648 hypothetical protein -
BJM00_RS21695 2890269..2890546 + 278 Protein_2649 type II toxin-antitoxin system VapB family antitoxin -
BJM00_RS14050 2890639..2891052 + 414 WP_003413164.1 PIN domain nuclease -
BJM00_RS14055 2891091..2891348 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BJM00_RS14060 2891345..2891722 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BJM00_RS14065 2891738..2892112 - 375 WP_003413177.1 hypothetical protein -
BJM00_RS14070 2892212..2892607 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BJM00_RS14075 2892604..2892849 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BJM00_RS14085 2893260..2893679 - 420 WP_003413190.1 A24 family peptidase -
BJM00_RS14090 2893691..2894500 - 810 WP_003413193.1 shikimate dehydrogenase -
BJM00_RS14095 2894497..2895750 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BJM00_RS14100 2895743..2896255 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T67543 WP_003413180.1 NZ_CP017594:c2892607-2892212 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T67543 NZ_CP017594:c2892607-2892212 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT67543 WP_003413183.1 NZ_CP017594:c2892849-2892604 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT67543 NZ_CP017594:c2892849-2892604 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References