Detailed information of TA system TA067540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2876752..2877392
• Replicon: chromosome
• Accession: NZ_CP017594
• Organism: Mycobacterium tuberculosis strain Beijing-like/36918

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: BJM00_RS13960
• Protein ID: WP_003412970.1
• Coordinates: 2876752..2877171 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: BJM00_RS13965
• Protein ID: WP_003412975.1
• Coordinates: 2877168..2877392 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJM00_RS13930	2872342..2873064	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
BJM00_RS13940	2873581..2873803	+	223	Protein_2628	type II toxin-antitoxin system VapB family antitoxin	-
BJM00_RS13945	2873800..2874201	+	402	WP_003412963.1	PIN domain-containing protein	-
BJM00_RS13950	2874236..2875156	-	921	WP_003412965.1	restriction endonuclease	-
BJM00_RS22065	2875497..2875742	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
BJM00_RS13955	2875801..2876751	+	951	WP_003911916.1	Lsr2 family protein	-
BJM00_RS13960	2876752..2877171	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
BJM00_RS13965	2877168..2877392	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
BJM00_RS13970	2877423..2880266	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
BJM00_RS13975	2880338..2880739	-	402	WP_003412981.1	hypothetical protein	-
BJM00_RS13980	2880739..2881209	-	471	WP_003899364.1	transcription antitermination factor NusB	-
BJM00_RS13985	2881212..2881775	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T67540 WP_003412970.1 NZ_CP017594:c2877171-2876752 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T67540 NZ_CP017594:c2877171-2876752 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT67540 WP_003412975.1 NZ_CP017594:c2877392-2877168 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT67540 NZ_CP017594:c2877392-2877168 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09