Detailed information of TA system TA067534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2277482..2278108
• Replicon: chromosome
• Accession: NZ_CP017594
• Organism: Mycobacterium tuberculosis strain Beijing-like/36918

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: BJM00_RS11015
• Protein ID: WP_003410075.1
• Coordinates: 2277710..2278108 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: BJM00_RS11010
• Protein ID: WP_003911750.1
• Coordinates: 2277482..2277709 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJM00_RS11000	2275521..2275865	-	345	WP_003410065.1	ferredoxin family protein	-
BJM00_RS11005	2276054..2277223	-	1170	WP_003899126.1	ATP-binding protein	-
BJM00_RS11010	2277482..2277709	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BJM00_RS11015	2277710..2278108	+	399	WP_003410075.1	PIN domain nuclease	Toxin
BJM00_RS11020	2278291..2278722	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
BJM00_RS11025	2278823..2279257	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
BJM00_RS11040	2279694..2279873	-	180	Protein_2093	hypothetical protein	-
BJM00_RS11045	2279934..2280581	+	648	WP_071854215.1	transposase	-
BJM00_RS11050	2280535..2281125	+	591	WP_003899131.1	IS110 family transposase	-
BJM00_RS11055	2281253..2282509	-	1257	WP_003902247.1	HNH endonuclease	-
BJM00_RS11060	2282863..2283063	+	201	Protein_2097	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T67534 WP_003410075.1 NZ_CP017594:2277710-2278108 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T67534 NZ_CP017594:2277710-2278108 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT67534 WP_003911750.1 NZ_CP017594:2277482-2277709 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT67534 NZ_CP017594:2277482-2277709 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8