Detailed information of TA system TA067519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1241725..1242293
• Replicon: chromosome
• Accession: NZ_CP017594
• Organism: Mycobacterium tuberculosis strain Beijing-like/36918

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: BJM00_RS06115
• Protein ID: WP_003405865.1
• Coordinates: 1241919..1242293 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: BJM00_RS06110
• Protein ID: WP_003405863.1
• Coordinates: 1241725..1241922 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJM00_RS06090	1237766..1238404	-	639	WP_003898730.1	lipid droplet-associated protein	-
BJM00_RS06095	1238494..1239501	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BJM00_RS06100	1239518..1240501	-	984	WP_003898731.1	hypothetical protein	-
BJM00_RS06105	1240564..1241637	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
BJM00_RS06110	1241725..1241922	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BJM00_RS06115	1241919..1242293	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
BJM00_RS06120	1242496..1243194	+	699	WP_003898733.1	hypothetical protein	-
BJM00_RS06125	1243312..1243497	+	186	WP_003901093.1	hypothetical protein	-
BJM00_RS06130	1243424..1243699	-	276	WP_031651777.1	hypothetical protein	-
BJM00_RS06135	1243942..1244265	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
BJM00_RS06140	1244280..1244990	-	711	WP_003911399.1	hypothetical protein	-
BJM00_RS21935	1245012..1245221	+	210	WP_003911400.1	hypothetical protein	-
BJM00_RS06145	1245173..1245880	-	708	Protein_1165	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T67519 WP_003405865.1 NZ_CP017594:1241919-1242293 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T67519 NZ_CP017594:1241919-1242293 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT67519 WP_003405863.1 NZ_CP017594:1241725-1241922 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT67519 NZ_CP017594:1241725-1241922 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07