Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2878381..2879018 Replicon chromosome
Accession NZ_CP017593
Organism Mycobacterium tuberculosis strain Beijing-like/35049

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BJL99_RS14010 Protein ID WP_003413180.1
Coordinates 2878381..2878776 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BJL99_RS14015 Protein ID WP_003413183.1
Coordinates 2878773..2879018 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJL99_RS13965 2873784..2874995 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BJL99_RS13975 2875122..2875781 + 660 WP_003902296.1 hypothetical protein -
BJL99_RS13980 2875778..2876441 + 664 Protein_2644 hypothetical protein -
BJL99_RS21655 2876438..2876715 + 278 Protein_2645 type II toxin-antitoxin system VapB family antitoxin -
BJL99_RS13990 2876808..2877221 + 414 WP_003413164.1 PIN domain nuclease -
BJL99_RS13995 2877260..2877517 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BJL99_RS14000 2877514..2877891 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BJL99_RS14005 2877907..2878281 - 375 WP_003413177.1 hypothetical protein -
BJL99_RS14010 2878381..2878776 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BJL99_RS14015 2878773..2879018 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BJL99_RS14025 2879429..2879848 - 420 WP_003413190.1 A24 family peptidase -
BJL99_RS14030 2879860..2880669 - 810 WP_003413193.1 shikimate dehydrogenase -
BJL99_RS14035 2880666..2881919 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BJL99_RS14040 2881912..2882424 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T67483 WP_003413180.1 NZ_CP017593:c2878776-2878381 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T67483 NZ_CP017593:c2878776-2878381 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT67483 WP_003413183.1 NZ_CP017593:c2879018-2878773 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT67483 NZ_CP017593:c2879018-2878773 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References