Detailed information of TA system TA067445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 701451..702097
• Replicon: chromosome
• Accession: NZ_CP017593
• Organism: Mycobacterium tuberculosis strain Beijing-like/35049

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: BJL99_RS03205
• Protein ID: WP_003403137.1
• Coordinates: 701451..701864 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: BJL99_RS03210
• Protein ID: WP_003403139.1
• Coordinates: 701861..702097 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJL99_RS03185	697534..699084	+	1551	WP_003905349.1	MCE family protein	-
BJL99_RS03190	699136..699528	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
BJL99_RS03195	699525..699782	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
BJL99_RS03200	699965..701200	-	1236	WP_003403128.1	ATP-binding protein	-
BJL99_RS03205	701451..701864	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
BJL99_RS03210	701861..702097	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BJL99_RS03215	702201..702707	-	507	WP_003900973.1	two component system sensor kinase HK1	-
BJL99_RS03220	702821..703351	-	531	WP_003917320.1	two component system sensor kinase HK2	-
BJL99_RS03225	703335..704030	-	696	WP_003918099.1	two-component system response regulator TcrA	-
BJL99_RS03230	704153..704464	+	312	WP_003403164.1	hypothetical protein	-
BJL99_RS03235	704536..705486	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
BJL99_RS03240	705727..706311	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
BJL99_RS03245	706313..707023	+	711	Protein_630	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T67445 WP_003403137.1 NZ_CP017593:c701864-701451 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T67445 NZ_CP017593:c701864-701451 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT67445 WP_003403139.1 NZ_CP017593:c702097-701861 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT67445 NZ_CP017593:c702097-701861 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89