Detailed information of TA system TA067434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 96469..97094
• Replicon: plasmid pDSJ09
• Accession: NZ_CP017590
• Organism: Pantoea stewartii subsp. stewartii DC283

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DSJ_RS25225
• Protein ID: WP_006122272.1
• Coordinates: 96696..97094 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H3RL78
• Locus tag: DSJ_RS25220
• Protein ID: WP_006122273.1
• Coordinates: 96469..96696 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSJ_RS25185	91934..92311	+	378	WP_006122276.1	hypothetical protein	-
DSJ_RS25190	92394..93425	+	1032	WP_044241399.1	IS630 family transposase	-
DSJ_RS25195	93705..94382	+	678	WP_044243389.1	hypothetical protein	-
DSJ_RS25200	94748..95254	-	507	WP_100208653.1	type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB	-
DSJ_RS25210	95645..96088	-	444	WP_044243383.1	hypothetical protein	-
DSJ_RS25215	96075..96374	-	300	WP_100208652.1	hypothetical protein	-
DSJ_RS25220	96469..96696	+	228	WP_006122273.1	antitoxin	Antitoxin
DSJ_RS25225	96696..97094	+	399	WP_006122272.1	tRNA(fMet)-specific endonuclease VapC	Toxin
DSJ_RS25230	97096..97890	-	795	WP_006122271.1	type-F conjugative transfer system pilin assembly protein TraF	-
DSJ_RS25235	97887..98246	-	360	WP_080577513.1	conjugal transfer protein TraN	-
DSJ_RS25240	98209..99744	-	1536	WP_006122269.1	IS66 family transposase	-
DSJ_RS25245	99764..100111	-	348	WP_006117592.1	IS66 family insertion sequence element accessory protein TnpB	-
DSJ_RS25250	100108..100737	-	630	WP_044241253.1	IS66 family insertion sequence hypothetical protein	-
DSJ_RS25255	100768..101442	-	675	WP_006122268.1	type-F conjugative transfer system secretin TraK	-
DSJ_RS25260	101429..101995	-	567	WP_006122267.1	type IV conjugative transfer system protein TraE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..132938	132938

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-74)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 17065.17 Da
• Isoelectric Point: 12.9205

>T67434 WP_006122272.1 NZ_CP017590:96696-97094 [Pantoea stewartii subsp. stewartii DC283]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 399 bp

>T67434 NZ_CP017590:96696-97094 [Pantoea stewartii subsp. stewartii DC283]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 9759.25 Da
• Isoelectric Point: 12.6750

>AT67434 WP_006122273.1 NZ_CP017590:96469-96696 [Pantoea stewartii subsp. stewartii DC283]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 228 bp

>AT67434 NZ_CP017590:96469-96696 [Pantoea stewartii subsp. stewartii DC283]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Antitoxin

• AlphaFold DB: H3RL78