Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1450561..1451209 Replicon chromosome
Accession NZ_CP017543
Organism Mannheimia haemolytica strain 1383

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG611_RS07355 Protein ID WP_006251221.1
Coordinates 1450561..1450737 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG611_RS07360 Protein ID WP_006251222.1
Coordinates 1450793..1451209 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG611_RS07330 1447711..1448169 - 459 WP_006251218.1 hypothetical protein -
BG611_RS07335 1448338..1448538 + 201 WP_142778547.1 hypothetical protein -
BG611_RS07345 1448636..1449676 + 1041 WP_015587043.1 IS481 family transposase -
BG611_RS07350 1449794..1450465 - 672 WP_006251220.1 hypothetical protein -
BG611_RS07355 1450561..1450737 + 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG611_RS07360 1450793..1451209 + 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG611_RS07365 1451249..1451731 - 483 WP_006251223.1 phage tail protein -
BG611_RS07370 1451735..1452115 - 381 WP_006251224.1 hypothetical protein -
BG611_RS07375 1452112..1452480 - 369 WP_006251225.1 hypothetical protein -
BG611_RS07380 1452482..1452826 - 345 WP_006251226.1 hypothetical protein -
BG611_RS07385 1452826..1453203 - 378 WP_006251227.1 hypothetical protein -
BG611_RS14010 1453206..1453493 - 288 WP_186806282.1 hypothetical protein -
BG611_RS07390 1453505..1454494 - 990 WP_006251229.1 encapsulin -
BG611_RS07395 1454509..1454943 - 435 WP_006251230.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1432263..1487718 55455
- flank IS/Tn - - 1448636..1449676 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67344 WP_006251221.1 NZ_CP017543:1450561-1450737 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67344 NZ_CP017543:1450561-1450737 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67344 WP_006251222.1 NZ_CP017543:1450793-1451209 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67344 NZ_CP017543:1450793-1451209 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References