Detailed information of TA system TA066878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1208049..1208706
• Replicon: chromosome
• Accession: NZ_CP017481
• Organism: Pectobacterium polaris strain NIBIO1006

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BJJ97_RS05470
• Protein ID: WP_095993311.1
• Coordinates: 1208335..1208706 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: BJJ97_RS05465
• Protein ID: WP_010281167.1
• Coordinates: 1208049..1208348 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJJ97_RS05445	1203284..1204291	+	1008	WP_095993308.1	nucleoside hydrolase	-
BJJ97_RS05450	1204291..1205268	+	978	WP_095993309.1	ABC transporter permease	-
BJJ97_RS05455	1205265..1206173	+	909	WP_095701024.1	ABC transporter permease	-
BJJ97_RS05460	1206213..1208012	+	1800	WP_095993310.1	ABC transporter ATP-binding protein	-
BJJ97_RS05465	1208049..1208348	-	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
BJJ97_RS05470	1208335..1208706	-	372	WP_095993311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BJJ97_RS05475	1209044..1209742	+	699	WP_039485183.1	D-galactonate utilization transcriptional regulator DgoR	-
BJJ97_RS05480	1209739..1210635	+	897	WP_095993312.1	2-dehydro-3-deoxygalactonokinase	-
BJJ97_RS05485	1210619..1211236	+	618	WP_095993313.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
BJJ97_RS05490	1211233..1212381	+	1149	WP_005976723.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14111.01 Da
• Isoelectric Point: 4.8476

>T66878 WP_095993311.1 NZ_CP017481:c1208706-1208335 [Pectobacterium polaris]
MAWEIVTRALFDIWFDEQTEAVQEEVLAHLAILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVGGHPYRTFFAFDRTRKA
IILCAGDKKGEDEKLFYEHMIKIADAEFTSHLISPEAKEDGNA

Nucleotide

• Length: 372 bp

>T66878 NZ_CP017481:c1208706-1208335 [Pectobacterium polaris]
TTGGCATGGGAAATAGTCACACGGGCGCTATTCGATATTTGGTTTGATGAACAAACTGAAGCGGTACAGGAAGAGGTTTT
GGCACATCTCGCTATACTCGAAGAGGATGGCCCACAGCTAGGACGACCACAGGTTGATCAGATTAAAGGCTCAAAATACA
AAAATATGAAGGAGCTTCGGGTTCAGGTTGGCGGCCACCCATATCGAACATTCTTCGCGTTTGACCGGACACGTAAGGCG
ATTATTTTATGTGCGGGAGATAAGAAAGGCGAAGATGAAAAATTGTTCTACGAACATATGATTAAGATCGCAGACGCTGA
GTTTACATCGCATCTGATATCACCGGAGGCAAAAGAAGATGGCAACGCTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT66878 WP_010281167.1 NZ_CP017481:c1208348-1208049 [Pectobacterium polaris]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT66878 NZ_CP017481:c1208348-1208049 [Pectobacterium polaris]
ATGGCAACGCTTAAAGAAATGATGGCTAAACGTTCACCAGAGAGCCGCGCGCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAAGAAATCACTTTAGCGAAACTACGCGAAGAGCTGAACATGTCGCAGACTCAGCTTGCGTTAGCTCTGGGCGTCAAAC
AGCCATCCATTGTTAAGATGGAGAAGGTCGAGAATGACCCAAAACTGTCAACGCTGAAACGCTATGTCACTGCCCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGTAAGCGTATCGCGCTTACTTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A433N287