Detailed information of TA system TA066542

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2891790..2892015
• Replicon: chromosome
• Accession: NZ_CP017434
• Organism: Escherichia coli O157:H7 strain 1130

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: A4C50_RS14875
• Protein ID: WP_000813258.1
• Coordinates: 2891790..2891945 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2891957..2892015 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4C50_RS14825	2886792..2887220	-	429	WP_001303509.1	tellurite resistance protein	-
A4C50_RS14840	2887675..2888388	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
A4C50_RS31050	2888524..2888721	-	198	WP_000917763.1	hypothetical protein	-
A4C50_RS14850	2888946..2889500	-	555	WP_000640035.1	DUF1133 family protein	-
A4C50_RS14855	2889563..2889868	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
A4C50_RS14860	2889881..2890930	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
A4C50_RS14865	2890932..2891204	-	273	WP_000191872.1	hypothetical protein	-
A4C50_RS14870	2891326..2891670	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
A4C50_RS14875	2891790..2891945	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2891957..2892015	+	59	-	-	Antitoxin
A4C50_RS14880	2892236..2892793	-	558	WP_000104474.1	DUF551 domain-containing protein	-
A4C50_RS14885	2892795..2893013	-	219	WP_000683609.1	DUF4014 family protein	-
A4C50_RS14890	2893141..2893452	-	312	WP_001289673.1	hypothetical protein	-
A4C50_RS14895	2893445..2893672	-	228	WP_000699809.1	hypothetical protein	-
A4C50_RS14900	2893669..2893950	-	282	WP_000603384.1	DNA-binding protein	-
A4C50_RS14905	2893983..2894699	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
A4C50_RS31055	2894733..2895194	-	462	WP_000139447.1	replication protein	-
A4C50_RS31060	2895187..2896230	-	1044	WP_001262402.1	hypothetical protein	-
A4C50_RS14920	2896299..2896724	-	426	WP_000693878.1	Rha family transcriptional regulator	-
A4C50_RS14925	2896708..2896950	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / paa	2854698..2967220	112522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T66542 WP_000813258.1 NZ_CP017434:c2891945-2891790 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T66542 NZ_CP017434:c2891945-2891790 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT66542 NZ_CP017434:2891957-2892015 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin