Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3720725..3721315 Replicon chromosome
Accession NZ_CP017289
Organism Klebsiella variicola strain GJ3

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag BBD65_RS20540 Protein ID WP_008804165.1
Coordinates 3720725..3721057 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag BBD65_RS20545 Protein ID WP_012541132.1
Coordinates 3721058..3721315 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBD65_RS20515 3716763..3717008 + 246 WP_008804171.1 signal transduction protein PmrD -
BBD65_RS20520 3717139..3718593 + 1455 WP_008804170.1 AMP nucleosidase -
BBD65_RS20530 3718966..3720402 - 1437 WP_162494461.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
BBD65_RS20540 3720725..3721057 - 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BBD65_RS20545 3721058..3721315 - 258 WP_012541132.1 antitoxin Antitoxin
BBD65_RS20550 3721844..3722944 + 1101 WP_048268588.1 phosphotransferase -
BBD65_RS20555 3722941..3723912 - 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
BBD65_RS20560 3724118..3724954 - 837 WP_008804155.1 alpha/beta hydrolase -
BBD65_RS20565 3725131..3725706 - 576 WP_077139353.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T66414 WP_008804165.1 NZ_CP017289:c3721057-3720725 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T66414 NZ_CP017289:c3721057-3720725 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACTCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCAGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT66414 WP_012541132.1 NZ_CP017289:c3721315-3721058 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT66414 NZ_CP017289:c3721315-3721058 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References