Detailed information of TA system TA066256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 48273..48795
• Replicon: plasmid pFAM21845_2
• Accession: NZ_CP017222
• Organism: Escherichia coli strain FAM21845

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: BHT24_RS27040
• Protein ID: WP_000220561.1
• Coordinates: 48514..48795 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: BHT24_RS27035
• Protein ID: WP_000121743.1
• Coordinates: 48273..48524 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHT24_RS27000	43502..43732	+	231	WP_000051065.1	partitioning protein	-
BHT24_RS27005	43748..44059	+	312	WP_000360321.1	hypothetical protein	-
BHT24_RS27010	44097..44717	+	621	WP_000342587.1	hypothetical protein	-
BHT24_RS27015	44876..45364	+	489	WP_001567305.1	hypothetical protein	-
BHT24_RS27020	45395..45817	-	423	WP_012845383.1	DNA distortion polypeptide 3	-
BHT24_RS27025	45885..46331	-	447	WP_001074386.1	hypothetical protein	-
BHT24_RS27030	46356..47210	-	855	WP_001239324.1	replication initiation protein	-
BHT24_RS27035	48273..48524	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
BHT24_RS27040	48514..48795	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BHT24_RS27045	49145..49660	+	516	WP_001025387.1	J domain-containing protein	-
BHT24_RS27050	49734..50282	+	549	WP_001178641.1	hypothetical protein	-
BHT24_RS27055	50348..50560	+	213	WP_001446884.1	hypothetical protein	-
BHT24_RS27060	50550..50792	+	243	WP_000008708.1	hypothetical protein	-
BHT24_RS27065	50886..51215	+	330	WP_000866654.1	hypothetical protein	-
BHT24_RS28105	51507..51653	+	147	WP_001567307.1	hypothetical protein	-
BHT24_RS27080	51670..51942	+	273	WP_000167280.1	hypothetical protein	-
BHT24_RS28110	52199..52273	+	75	Protein_57	DNA distortion polypeptide 3	-
BHT24_RS27085	52268..52813	+	546	WP_001426313.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1A	mrkA / mrkB / mrkC / mrkD / mrkF	1..54159	54159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11023.86 Da
• Isoelectric Point: 10.5938

>T66256 WP_000220561.1 NZ_CP017222:48514-48795 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T66256 NZ_CP017222:48514-48795 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT66256 WP_000121743.1 NZ_CP017222:48273-48524 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT66256 NZ_CP017222:48273-48524 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301