Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2281970..2282472 Replicon chromosome
Accession NZ_CP017220
Organism Escherichia coli strain FAM21845

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BHT24_RS12135 Protein ID WP_000767829.1
Coordinates 2281970..2282224 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BHT24_RS12140 Protein ID WP_001259255.1
Coordinates 2282221..2282472 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHT24_RS12110 2276985..2277212 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
BHT24_RS12115 2277226..2278284 - 1059 WP_000492320.1 transport protein YeeE -
BHT24_RS12120 2278463..2279821 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BHT24_RS27465 2279811..2279873 - 63 WP_010723108.1 membrane protein YoeI -
BHT24_RS12125 2280088..2281017 - 930 WP_081178150.1 LysR family transcriptional regulator -
BHT24_RS12130 2281063..2281887 - 825 WP_000754737.1 SDR family oxidoreductase -
BHT24_RS12135 2281970..2282224 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BHT24_RS12140 2282221..2282472 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BHT24_RS12145 2282755..2282805 + 51 WP_001368802.1 his operon leader peptide -
BHT24_RS12155 2282951..2283850 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
BHT24_RS12160 2283856..2285160 + 1305 WP_016239568.1 histidinol dehydrogenase -
BHT24_RS12165 2285157..2286227 + 1071 WP_000108952.1 histidinol-phosphate transaminase -
BHT24_RS12170 2286227..2287294 + 1068 WP_081178151.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T66240 WP_000767829.1 NZ_CP017220:c2282224-2281970 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T66240 NZ_CP017220:c2282224-2281970 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT66240 WP_001259255.1 NZ_CP017220:c2282472-2282221 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT66240 NZ_CP017220:c2282472-2282221 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References