Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3563702..3564372 Replicon chromosome
Accession NZ_CP016972
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag BB988_RS17560 Protein ID WP_003899954.1
Coordinates 3563702..3564046 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag BB988_RS17565 Protein ID WP_003899955.1
Coordinates 3564043..3564372 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB988_RS17525 3558775..3559635 + 861 WP_003416628.1 alpha/beta hydrolase -
BB988_RS17530 3559610..3560125 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
BB988_RS17540 3560365..3560658 + 294 WP_003416635.1 hypothetical protein -
BB988_RS17545 3560946..3562235 + 1290 WP_003416640.1 ATP-binding protein -
BB988_RS17550 3562582..3563016 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
BB988_RS17555 3563019..3563471 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BB988_RS17560 3563702..3564046 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BB988_RS17565 3564043..3564372 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
BB988_RS17575 3564734..3565995 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
BB988_RS17580 3566268..3566615 + 348 WP_003899956.1 DUF2384 domain-containing protein -
BB988_RS17585 3566612..3567232 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
BB988_RS17590 3567392..3568657 - 1266 WP_003912135.1 hypothetical protein -
BB988_RS17595 3568825..3569034 + 210 WP_003416778.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T65765 WP_003899954.1 NZ_CP016972:3563702-3564046 [Mycobacterium tuberculosis H37Ra]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T65765 NZ_CP016972:3563702-3564046 [Mycobacterium tuberculosis H37Ra]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT65765 WP_003899955.1 NZ_CP016972:3564043-3564372 [Mycobacterium tuberculosis H37Ra]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT65765 NZ_CP016972:3564043-3564372 [Mycobacterium tuberculosis H37Ra]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References