Detailed information of TA system TA065765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3563702..3564372
• Replicon: chromosome
• Accession: NZ_CP016972
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: BB988_RS17560
• Protein ID: WP_003899954.1
• Coordinates: 3563702..3564046 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: BB988_RS17565
• Protein ID: WP_003899955.1
• Coordinates: 3564043..3564372 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB988_RS17525	3558775..3559635	+	861	WP_003416628.1	alpha/beta hydrolase	-
BB988_RS17530	3559610..3560125	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
BB988_RS17540	3560365..3560658	+	294	WP_003416635.1	hypothetical protein	-
BB988_RS17545	3560946..3562235	+	1290	WP_003416640.1	ATP-binding protein	-
BB988_RS17550	3562582..3563016	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
BB988_RS17555	3563019..3563471	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BB988_RS17560	3563702..3564046	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BB988_RS17565	3564043..3564372	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
BB988_RS17575	3564734..3565995	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-
BB988_RS17580	3566268..3566615	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
BB988_RS17585	3566612..3567232	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
BB988_RS17590	3567392..3568657	-	1266	WP_003912135.1	hypothetical protein	-
BB988_RS17595	3568825..3569034	+	210	WP_003416778.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T65765 WP_003899954.1 NZ_CP016972:3563702-3564046 [Mycobacterium tuberculosis H37Ra]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T65765 NZ_CP016972:3563702-3564046 [Mycobacterium tuberculosis H37Ra]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT65765 WP_003899955.1 NZ_CP016972:3564043-3564372 [Mycobacterium tuberculosis H37Ra]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT65765 NZ_CP016972:3564043-3564372 [Mycobacterium tuberculosis H37Ra]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6